ABSTRACT
ABSTRACT The tracheal pulmonary route is used in diverse experimental models for the study of drugs, infectious agents, and diseases. In view of its importance and associated difficulties, the present article proposes to give research groups up-to-date information on techniques to access the tracheal pulmonary pathway of small rodents.
RESUMO As vias de acesso traqueopulmonar vêm sendo utilizadas em diversos modelos experimentais que estudam a ação de fármacos e agentes infecciosos, além de enfermidades. Tendo em vista a sua importância e as dificuldades associadas, o presente artigo de atualização propõe-se a dar ao pesquisador as informações necessárias para o emprego das técnicas de acesso traqueopulmonar em pequenos roedores.
ABSTRACT
Murine normal lymphoid tissues contain relatively high numbers of anti-BrMRBC RFC and PFC. In the bone marrow, these frequencies are inversely correlated probably due to different activation states of these anti-BrMRBC B cells. The sera from syngeneic, but not allogeneic, mice are able to inhibit the formation of these anti-BrMRBC plaques. This inhibition is enhanced when sera of LPS treated mice is employed. The inhibitory factor can be displaced from normal spleen cells by 10(-5) M phosphorylcholine (PC) hapten reversing the PFC inhibition. The dialyzed and concentrated supernatant from PC incubated splenocytes completely abolishes anti-BrMRBC PFC. Preliminary data suggest that the molecular weight of the inhibitory factor is higher than 600 KDa. We postulate an antiidiotype autoantibody nature for this inhibitory factor.