ABSTRACT
The mosquito Aedes aegypti is the main vector of dengue in Venezuela. The genetic structure of this vector was investigated in 24 samples collected from eight geographic regions separated by up to 1160 km. We examined the distribution of a 359-basepair region of the NADH dehydrogenase subunit 4 mitochondrial gene among 1144 Ae. aegypti from eight collections. This gene was amplified by the polymerase chain reaction and tested for variation using single strand conformation polymorphism analysis. Seven haplotypes were detected throughout Venezuela and these were sorted into two clades. Significant differentiation was detected among collections and these were genetically isolated by distance.
Subject(s)
Animals , Aedes/genetics , DNA, Mitochondrial/genetics , Genetic Variation , Insect Vectors/genetics , NADH Dehydrogenase/genetics , Aedes/enzymology , Dengue/transmission , Geography , Haplotypes/genetics , Insect Vectors/enzymology , Polymerase Chain Reaction , Polymorphism, Single-Stranded Conformational , VenezuelaABSTRACT
Con el fin de introducir al tratamiento antimicótico un nuevo producto imidazólico de aplicación tópica, oxiconazol, se emprendió un estudio comparativo, abierto y longitudinal, comparando los efectos del citado compuesto versus ketoconazol, ambos en presentación de crema, para tratar a dos grupos de 15 pacientes cada uno, con diferentes formas clínicas de infestación micótica. En general, la eficacia de oxiconazol (1%) fue discretamente superior a la de ketoconazol (2%), aunque la diferencia observada no fue de significación. Ambos productos fueron bien tolerados, tanto tópica como sistémicamente