ABSTRACT
Androgenetic alopecia (AGA) is a common cause of hair loss in males and this has profound effect on the quality of life of patients. There are various treatment modalities and Platelet Rich Plasma (PRP) is one with promising effect in various studies. It contains platelets at a concentration of more than one million and contains various growth factors such as platelet-derived growth factors, vascular endothelial growth factors, epidermal growth factors which promote hair growth. As it is autologous, it has very few side effects.METHODS20 patients with AGA belonging to the age group 18- 40 years, Hamilton Norwood Stage 1-4 who attended dermatology OPD were included. Dermoscopic analysis of hair count was done at a specific distance from left medial eyebrow; the hair density was noted; images were taken and recorded. PRP was prepared by Manual Double Spin method; Under local anaesthesia, both spray technique and microdermabrasion injection technique were done. The procedure was repeated every 3 months and dermoscopy was done at each visit at the fixed site. Statistical analysis was done with ANOVA test and paired t test for these groups.RESULTS83% had decrease in hair fall and 72% reported increase in hair growth at 3 months. Pain was the most common side effect. There was significant increase in hair density at each visit and there was negative correlation between duration of disease and increase in hair density.
ABSTRACT
Vitiligo is an idiopathic, acquired, circumscribed, hypomelanotic skin disorder, characterized by depigmented or hypopigmented macules of different sizes and shapes. It is due to the destruction of melanocytes resulting in the absence of pigment production of the skin and mucosal surfaces. Oxidative stress has been implicated in pathophysiology of vitiligo. Oxidative stress reflects an imbalance between the production of reactive oxygen species and biological system's ability to readily detoxify the reactive intermediates or to repair the resulting damage.1 Malondialdehyde (MDA) is one of several low-molecular-weight end products formed via the decomposition of certain primary and secondary lipid peroxidation products. It is one of the important indicators of free radical-mediated tissue injury.METHODSIn this case control study, the serum MDA levels of 50 vitiligo patients were compared with 50 age and sex matched controls. Analysis was done in UV – Vis spectrophotometer and Malondialdehyde (MDA) was measured in the serum by a method based on Valipasha and Sadasivadu’s procedures for estimation of MDA.RESULTSMDA levels were high in cases and in patients with unstable and generalized vitiligo than in control group and stable and localized vitiligo with a significant p value. There was no significant gender difference in oxidative stress. This study concludes that oxidative stress has a significant role in development of vitiligo.CONCLUSIONSThere is oxidative stress in vitiligo as the serum level of malondialdehyde a marker of lipid peroxidation is elevated in vitiligo cases compared to controls.