ABSTRACT
Crustacean hyperglycemic hormone (CHH) family neuropeptides have been in research limelight for the past two decades due to their importance in the regulation of glycemia, moulting and gonad development in crustaceans. Under natural conditions, low levels of CHH neuropeptide and the structural similarity of the three CHH family neuropeptides limit their purification directly from the animal. In this study, we isolated the mature region of the CHH1 gene, constructed the recombinant translation expression vector (pET32a+ - PmCHH1) and produced thioredoxin fused protein in E scherichia coli (BL21 (DE3) pLysS). The translation expression vector construct (pET32a+ - PmCHH1) was successfully built for production of thioredoxin fused mature CHH1 protein (mf-PmCHH1-29.47 kDa). Mf-PmCHH1 produced a hyperglycemic effect similar to that of the eyestalk extract when experimentally injected into adult eyestalk ablated Penaeus monodon. The polyclonal antiserum (anti-mf-PmCHH1) was developed in mice against the purified thioredoxin fused mf-PmCHH1 protein. A hypoglycemic effect was induced in adult P. monodon by the polyclonal antiserum which was raised against thioredoxin fused CHH1 protein. Immunolocalization of CHH1 producing neurosecretory cells in the eyestalk of P. monodon was a practical result obtained with the polyclonal antiserum anti-mf-PmCHH1. Therefore, mf-PmCHH1 and its antiserum (anti-mf-PmCHH1) are added to the list of tools to better understand the endocrine mechanisms regulating glycemia and reproduction in P. Monodon
ABSTRACT
The phenomenon of scientific retraction is a shameful act for the scientific community, but a necessity to maintain the purity of science. The two main causes for retractions include plagiarism and research misconduct. The post retraction citation of articles is again a repetition of inappropriateness. Hence the editorial, peer review policies should be revised and the retractions should be publicized more to avoid the citation of the invalid literature. Also, the scientific readership has a responsibility to evaluate the scientific validity of published studies.
El fenómeno de la retractación científica constituye una vergüenza para la comunidad científica, pero es necesario para mantener la pureza de la ciencia. Las dos causas principales de retractación son el plagio y faltas en la conducta de investigación. La citación de artículos posterior a su retractación es, de nuevo, una repetición inapropiada. Por lo tanto, la editorial y las normas de evaluación por pares debieran revisarse y las retractaciones publicarse más para evitar las citaciones de literatura inválida. También los lectores científicos tienen la responsabilidad de evaluar la validez científica de los estudios publicados.
O fenômeno de retratação científica é uma vergonhosa atitude para a comunidade científica, mas uma necessidade para manter a pureza da ciência. As duas principais causas para a retratação incluem o plágio e a má conduta na pesquisa. A citação após retratação de artigos é novamente uma repetida inadequação. Daí porque o editorial e a política de revisão por pares devem ser revistas e as retratações deveriam ser mais divulgadas para evitar uma citação inválida da literatura. Ademais, o revisor científico tem uma responsabilidade de avaliar a validade científica dos estudos publicados.
Subject(s)
Scientific Misconduct/statistics & numerical data , Plagiarism , Retraction of Publication as TopicABSTRACT
Centella asiatica is a valuable medicinal plant with abundant amount of pharmaceutically useful compounds; however, it is important to know the proper physiological age for drug formulation. In the present study, smaller to larger thirteen groups of Centella asiatica leaves were evaluated for growth, chlorophyll content and antioxidant activity. The growth was measured in the terms of leaf area, fresh and dry weights and water content. Maximum antioxidant activity was found in third size group of the leaf which was further analyzed for phenolics in RP-HPLC. The probable role of these phenols in antioxidant activities is discussed.