1.
Egyptian Journal of Microbiology. 1984; 19 (1): 63-73
in English
| IMEMR
| ID: emr-94784
ABSTRACT
Pichia pastoris [704] was cultivated in fermenters either as fed-batch or continuous cultures using methanol as a sole source of carbon. Results revealed that the addition of microelements and vitamins in 4 equal portions [every 24 hr] to the fermenter increased the utilization efficiency of methanol. The tested yeast had a high affinity for methanol but a low affinity for formaldhyde and least affinity for formate. High low rates in continuous cultures led to a decrease the cell biomass in the fermenter. 02-uptake and C02 production were constant at each dilution rate [steady state] while in fed-batch cultures they gradually increased to reach their maximum at the end of exponential phase, then decrease due to the cessation of growth