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Objective@#To evaluate the effects of community health education on the quality of life of diabetic patients.@*Methods@#The databases of CNKI,Wanfang,CBMdisc,VIP,PubMed,Embase and Google Scholar were searched for the literatures about the effects of community health education on the quality of life of diabetic patients published from the time the databases established to August 1st,2019. Standardized mean difference（SMD）was used as a indicator for the meta-analysis. @*Results@#A total of 739 articles were retrieved,and 20 articles were finally included,with 1 727 cases in the experimental group and 1 645 cases in the control group. The results of the meta-analysis showed that compared with the traditional intervention methods and no intervention,community health education had obviously better effects on physiological function（SMD=0.67,95%CI：0.29-1.05,P<0.05）,psychological function（SMD=0.73,95%CI：0.40-1.06,P<0.05）and social relationship（SMD=0.69,95%CI：0.33-1.04,P<0.05）. The results were stable according to sensitivity analysis. No publication bias was found by Egger's test. @* Conclusion @# Community health education can effectively improve the quality of life of diabetic patients in physiological function,psychological function and social relationship.
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Objective@#To compare the osteogenic differentiation abilities of human bone marrow mesenchymal stem cells (hBMSCs) from different sources, and to provide basis for choosing a new source of seed cells in bone tissue engineering.@*Methods@# Jaw bone-marrow-derived mesenchymal stem cells (JMMSCs) were isolated from orthognathic surgical sites and cultured by limited dilution for single cell clone. Long bone-marrow-derived mesenchymal stem cells (BMMSCs) were obtained from bone marrow of volunteers and isolated by density gradient centrifugation method. Flow cytometry was used to detect the surface markers of both cells. Osteogenic ability was assessed by PCR and Western Blot after osteogenic differentiation for the following molecules: Runx2, COL-1 and OCN. Alizarin red staining was used for determining the ability of cell mineralization after osteogenic differentiation. @*Results @#The expressions of cell surface markers CD90 and CD105 were positive in both type of cells, while CD34, CD14 and CD45 were all negative. After 21 days of osteogenic induction, JMMSCs formed significantly more mineralized nodules than BMMSCs. After 7, 14, 21 days of osteogenic induction, JMMSCs expressed more osteogenic-related molecules than BMMSCs.@*Conclusion@#The osteogenic differentiation capacity and mineralization ability of JMMSCs are significantly higher than BMMSCs. Jaw bone might be a more suitable source of seed cells in bone tissue engineering compared with long bone.
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Objective To improve the buffer for washing frozen red blood cells(RBCs)and explore the feasibility of replacing the middle buffer of 2% NaCl with 0.9% NaCl solution in the protocol of deglycerolization and the effect was evaluated.Methods Two units of RBCs separated from whole blood of healthy donors were frozen at -80℃.The thawed frozen RBCs were treated with a washing machine.On the basis of the old protocol(protocol 1),the middle buffer of 2%NaCl was replaced,and the usage of washing buffer and the washing steps were adjusted to create a new protocol(protocol 2).The quality of RBCs washed by the two protocols was evaluated.Results According to the results of the detection of RBCs treated by protocol 1 and 2: the hemoglobin contents(g)were 42.18 ±3.35 and 44.98 ±1.68,respectively,free hemoglobin contents(g/L)were 0.53 ±0.06 and 0.45 ±0.05 respectively, the residual amount of white blood cells (×107)was 1.92 ±1.04 and 1.12 ±1.12,and the osmolarities(mOsm)were 327.0 ±9.06 and 331.8 ±10.62 respectively. Sterile experiments were negative for both bacteria and fungi.The hemolysis rates(%)were 12.02 ±5.78 and 14.30 ± 5.67 respectively.The deforming abilities(%)were 21.42 ±1.45 and 21.32 ±0.84 respectively.The RBC recoveries (%)were 77.18 ±5.58 and 79.63 ±2.06 respectively.The processing time(min)was 79.60 ±0.55 and 78.80 ±1.30 respectively.There was no significant difference in the quality of frozen RBCs washed by the old protocol(protocol 1)and the new protocol(protocol 2)(P>0.05).Conclusion The quality of frozen RBCs washed by the two protocols meet national standards.
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Objective To construct the recombinant adenovirus vector carrying mouse β-nerve growth factor (β-NGF) labeled with green fluorescent protein (GFP). Methods Adult healthy male Kunming mice, weighting (60-70) g, were chosen in our study. The full-length β-NGF cDNA was amplified from the total mRNA of mouse submandibular gland by RT-PCR, and then, this fragment was cloned into the adenovirus shuttle plasmid pAdtrack-CMV (marked with GFP); and then, the pAdeasy-1/pAdtrack-CMV-β-NGF was constructed with defective adenovirus genome pAdeasy-1 by homologous recombination in bacteria; at last, restriction analysis was performed on this adenovirus vector pAdeasy-l/pAdtrack-CMV-GFP-β-NGF.Results Amplified full-length mouse β-NGF fragment was obtained by RT-PCR; the pAdTrack-CMV-β-NGF contained the target gene; double digestion indicated that the recombinant plasmid pAdTrack-CMV-β-NGF was successfully constructed;restriction enzyme digestion analysis indicated that pAdeasy-l/pAdtrack-CMV-βNGF adenovirus vector by homologous recombination was constructed. Conclusion The recombinant adenovirus vector pAdeasy- 1/pAdtrack-CMV-GFP-β-NGF is successfully constructed.
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Objective To investigate the effect of endovascular interventional therapy on intracranial aneurysm.Methods We retrospectively analyzed the clinical information and treatment efficacy of 48 patients (53 aneurysms) experienced interventional embolism therapy; these patients were admitted to and received treatment in our hospital from January 2001 to December 2009.Results Among the 53 aneurysms of 48 patients,40 aneurysms were obliterated completely,6 aneurysms 95％ obliterated,5 aneurysms 90％ obliterated and 2 aneurysms failed; 2 aneurysms ruptured and no death was noted during the operation.Six to 12 months after the operation,follow-up of the 46 patients indicated that 2 were recurred under CMA or DSA; 2 was severely disabled; 5 had mild neurological deficits; and the other enjoyed good results.Conclusion Endovascular embolization ofaneurysms is a minimally invasive method with low risk; individualized embolism therapy can improve the prognosis.
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Objective To explore the possibility of BMSCs differentiating into neurons under thc condition of in vitro culture by striatal extracts (SEs) from 6-hydroxydopamine (6-OHDA)-induced PD rats. Methods PD rat models were established by stereotaxic injection of 6-OHDA into the right medial forebrain bundle. Bilateral striatum was dissected; lesion striatal extracts (L-SEs) and intact striatal extracts (I-SEs) were prepared. The in vitro isolated and expanded third-passage BMSCs were induced by the 2 striatal extracts, respectively. Cells were observed under phase contrast microscope. The differentiated cells were detected and measured by immunocytochemical stain with nestin, neuron special enolase (NSE), glial fibrillary acidic protein (GFAP) and tyroxine hydroxylase (TH). Besides that, some other BMSCs were cultured in the serumal culture solution (Ser-C) and free serum culture solution (F-SerC) as controls. Results Morphological changes of BMSCs after treatment could be observed under contrast phase microscope. Some cells even had enations and some cells in the L-SEs and I-SEs inducement groups were out of shape. Cells in the Ser-C treatment group grew well, while cells in F-SerC treatment group could not grow well. Immunohistochemical staining indicated that GFAP positive cells expressed in the Ser-C treatment group; the GFAP and nestin positive cells in the L-SEs inducement group were increased and the GFAP and NSE positive cells in the L-SEs inducement group were increased as compared with those in the Ser-C treatment group; furthermore, the percentages of GFAP positive cells in the L-SEs and I-SEs inducement groups (53.09±32.27%, 54.60±33.14%) were significantly different with those in the Ser-C treatment group (15.05±3.92%, P＜0.05) and those were not obviously different between the L-SEs and I-SEs inducement groups (P＞0.05). No TH positive cells existed in all the groups. Conclusion SEs from 6-OHDA-induced PD rats can lead to morphological changes of BMSCs, and some cells have enations. A certain number of cells express neural special proteins after inducemet, including GFAP positive cells and some nestin/NSE positive cells.