Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 17 de 17
Filter
1.
Braz. J. Pharm. Sci. (Online) ; 56: e18482, 2020. graf
Article in English | LILACS | ID: biblio-1249142

ABSTRACT

Up to date, the management of hepatotoxicity induced by a suicidal or unintentional overdose of acetaminophen (APAP) remains a therapeutic challenge. The present study aimed to elucidate the potential effect of sitagliptin, a DPP-4 inhibitor, to ameliorate the acute injurious effects of acetaminophen on the liver. APAP toxicity was induced in mice by an intraperitoneal injection of APAP (400 mg/kg). The effect of treatment with sitagliptin, initiated 5 days prior to APAP injection, was evaluated. Serum indices of hepatotoxicity, oxidative stress markers in liver tissues, serum IL-1ß, and TNF-α in addition to hepatic- NF-E2-related factor-2 (Nrf2) were determined. Our results showed that APAP induced marked hepatic injury as evidenced by an increase in serum levels of ALT and AST, in addition to the deterioration of histological grading. Oxidative stress markers, serum TNF-α, and IL-1ß were also elevated. Sitagliptin successfully ameliorated the histological changes induced by APAP, improving liver function tests and liver oxidant status accompanied with a marked increase in Nrf2 level in hepatic tissues. Thus, the hepatoprotective effects of sitagliptin in this animal model seem to involve Nrf2 modulation, coincidental with its anti-inflammatory and antioxidant effects


Subject(s)
Animals , Male , Mice , Therapeutics/adverse effects , Sitagliptin Phosphate/analysis , Acetaminophen/adverse effects , Wounds and Injuries/classification , Oxidative Stress , Models, Animal , Dipeptidyl-Peptidase IV Inhibitors , Liver/abnormalities , Liver Function Tests , Antioxidants/administration & dosage
2.
EJMM-Egyptian Journal of Medical Microbiology [The]. 2015; 24 (3): 27-35
in English | IMEMR | ID: emr-169568

ABSTRACT

The occurrence of a MbetaL-positive isolate in a hospital setting represents a therapeutic problem, as well as a serious concern for infection control management. The accurate identification and reporting of MbetaL-producing P. aeruginosa will aid infection control practitioners in preventing the spread of these multidrug-resistant isolates. This study aimed to detect and characterize MbetaL producing P. aeruginosa in Benha and to evaluate IMP- EDTA CDT as phenotypic screening method for MbetaL detection. This study was conducted on 100 P.aeruginosa strains isolated from 220 different clinical specimens collected from patients admitted to Benha University Hospital and Benha Teaching Hospital. The isolated P. aeruginosa strains were subjected to antibiotic susceptibility testing by disc diffusion test and MbetaL detection both phenotypically by IMP- EDTA CDT and genotypically by multiplex PCR. Out of 100 P.aeruginosa isolates, 25 strains [25%] were imipenem resistant and 15 strains of them [60%] were carrying genes responsible for MbetaL production [15% of the total number of P.aeruginosa]. Thirteen strains [13%] were carrying VIM gene while two strains [2%] were carrying both VIM and SPM genes together. IMP, GIM-1, SIM-1 genes were not detected. None of the imipenem sensitive strains were carrying genes of MbetaL production. Nearly all MbetaL producers were resistant to most antibiotics used while all strains were sensitive to colistin and polymyxinB. There is very good strength of agreement between IMP-EDTA CDT and PCR. The sensitivity and specificity of the IMPEDTA CDT in relation to PCR was 93.3% and 100% respectively. MbetaL producers is a serious problem as they are highly resistant to most antibiotics used making treatment options very limited, VIM gene is the most prevelant one in comparison with other genes of MbetaL production and IMP-EDTA CDT is a good and sensitive test in detecting MbetaL production

3.
EJMM-Egyptian Journal of Medical Microbiology [The]. 2015; 24 (3): 53-59
in English | IMEMR | ID: emr-169571

ABSTRACT

Human bocavirus [HBoV] infection possibly plays a role in gastroenteritis because of the frequent manifestation of gastrointestinal symptoms. Detect human bocavirus [HBoV] and assess its prevelance among gastroenteritis associated viral agents in infants with gastroenteritis in Benha University Hospital. The study was carried out on 100 stool samples collected from 100 infants with acute gastroenteritis for detection of Rotavirus [RV], Norovirus [NoV] and Astrovirus [AstV] by multiplex reverse transciptase polymerase chain reaction and detection of Adenovirus[AdV] and HBoV by multiplex polymerase chain reaction. Viral agents were detected in 57 [57%] samples; 51 [51%] samples show mono-infection while 6 [6%] samples show co-infection. Rotavirus, Norovirus, Adenovirus and astrovirus were detected in 37%, 14%, 7.0%, and 3% of the study population, respectively; HBoV was detected in 2%. This percentage of HBoV suggests that it might play a minor role in gastroenteritis

4.
New Egyptian Journal of Medicine [The]. 2009; 40 (1): 46-50
in English | IMEMR | ID: emr-92272

ABSTRACT

The evidence for an association between autoimmune forms of chronic hepatitis and hepatitis C virus [HCV] infection is controversial. Although anti-liver kidney microsomal antibody-positive autoimmune chronic hepatitis appears to be associated with HCV infection, evidence of such an association is less convincing with other subtypes of autoimmune chronic hepatitis. Autoantibodies are frequently found in patients with chronic hepatitis C, although usually in low titers which suggests that HCV elicits an immune response in the host. In a small subset of patient with chronic hepatitis C, autoantibodies are seen in high titers along with hypergammaglobulinemia, which further clouds the distinction between autoimmune chronic hepatitis and chronic hepatitis C.This has important therapeutic implications because inappropriate treatment of autoimmune chronic hepatitis with interferon-based therapy may exacerbate liver disease. Conversely, corticosteroid therapy for chronic hepatitis C may enhance HCV replication, which could worsen underlying liver disease. Few data are available to show how patients with features of both autoimmune hepatitis and chronic hepatitis C should be treated


Subject(s)
Humans , Hepatitis C, Chronic/diagnosis , Hepatitis C, Chronic/therapy , Diagnosis, Differential , Autoantibodies , Hypergammaglobulinemia , Adrenal Cortex Hormones , Interferons , Hepatitis, Autoimmune/therapy
5.
ASNJ-Alexandria Scientific Nursing Journal. 2009; 7 (1): 153-175
in English | IMEMR | ID: emr-97415

ABSTRACT

The aim of this study is to examine the effect of betadine versus chamomile on pain relief and healing process of episiotomy among primiparae. A sample of [90] postpartum women were selected from the post partum unit of Elshatby Maternity University Hospital in Alexandria. They were equally divided into two study groups. Each group was instructed to apply one of the intervention modalities: betadine or chamomile for ten days. Three tools were used for data collection. The first was a structured interview schedule to elicit the socio-demographic characteristics of the subjects. The 2nd tool was the healing and pain assessment scale which comprised 2 parts: the Reeda scale [RS] to evaluate the condition of the perineum before and after intervention; and the visual analoge scale [VAS] to assess the pain intensity before and after intervention. The 3rd tool was an observation follow up check list to assess the healing process of episiotomy among the two groups. The results revealed that women using chamomile significantly achieved complete or maximum recovery earlier and faster than women using betadine


Subject(s)
Humans , Female , Povidone-Iodine , Wound Healing , Pain/therapy , Chamomile/statistics & numerical data , Comparative Study
6.
New Egyptian Journal of Medicine [The]. 2007; 37 (4): 252-257
in English | IMEMR | ID: emr-172461

ABSTRACT

A 12-year-old otherwise healthy boy presented with hyperacute hepatic failure [encephalopathy, occurred within seven days of disease onset; total and direct bilirubin of 94 umol/l and 73 umol/I respectively; aspartate aminotransferase [AST] of 8,367 u/l; alanine aminotransferase [ALT] of 11,164 u/I; prolonged prothrombin time 29.1 sec [INR 3.03] and activated partial thromboplastin time of 48.2 sec [ratiol.6]], non-oliguric acute renal failure [creatinine of 591 umol/L and blood urea nitrogen of 21.0 mmol/L], transient leukopenia, transient thrombocytopenia, with new onset diabetes mellitus [on admission the patient had blood glucose of 23.9 mmol/L]. On admission, this patient had reactive 1gM antibody for acute hepatitis A with non-reactive results for hepatitis B surface antigen, hepatitis B core antibodies, and hepatitis C antibodies, The acute renal failure and hepatitis resolved in 17 days and 30 days from admission, respectively; but the patient continues to have diabetes mellitus 6 months [time of follow up] after the hepatitis A infection while the leukopenia and thrombocytopenia resolved in 3 and 6 days from admission respectively. This case illustrates unusual presentation of acute hepatitis A infection induced with acute liver failure, non-oliguric acute renal failure, and, diabetes mellitus as sequele of this infection


Subject(s)
Humans , Male , Diabetes Mellitus , Acute Kidney Injury , Liver Function Tests , Kidney Function Tests , Liver Failure , Follow-Up Studies
7.
Bulletin of Alexandria Faculty of Medicine. 2007; 43 (1): 7-11
in English | IMEMR | ID: emr-81991

ABSTRACT

This study aimed to evaluate the role of auto antibody profile in the diagnosis of patients with suspected autoimmune liver disease and their ability to define the putative type 3 autoimmune hepatitis [AIH]. Forty adult patients with abnormal liver function, elevated serum IgG and negative hepatitis markers were chosen from those attending the Hepatology Clinic and Internal Medicine Clinic of Alexandria Main University Hospital and Armed Forces Hospital. According to the results of liver biopsy, 36 patients were diagnosed as AIH, one patient as primary biliary cirrhosis [PBC] and the other 3 patients were still undiagnosed. Also, 10 patients with documented diagnosis of non-immune hepatitis B virus in addition to 10 age and sex matched healthy controls were enrolled in this study. Autoantibody profile of ANA, ASMA, AMA, LKM and p-ANCA was measured by indirect immunofluorescent technique [IF] and anti SLA/LP, LKM-1, LC-1 and AMA-M2 autoantibodies were detected by western blot assay. The sensitivity of ANA, ASMA, AMA, LKM, p-ANCA, SLA/LP and LC-1 were 48.6%, 51.4%, 2.7%, 8.1, 56.8%, 18.9% and 10.8% respectively with high specificity [100% for all auto-antibodies except that for ANA 95.7% and ASMA 87%]. However, the overall sensitivity of the complete profile was increased to 100% and specificity was 91.3% from this study we conclude that a complete profile of autoantibodies can be used as a useful tool for the diagnosis of patients with suspected autoimmune liver disease. Anti SLA/LP is an additional specific and diagnostic marker for the diagnosis of AIH type 1 and it remains to be seen whether the seropositivity of SLA/LP may characterize the patients who are more likely to relapse after corticosteroid therapy


Subject(s)
Humans , Hepatitis, Autoimmune/diagnosis , Autoantibodies , Antibodies, Antinuclear , Immunoglobulin G , Liver Function Tests , Biopsy , Liver , Histology , Liver Diseases/diagnosis
8.
Alexandria Journal of Pediatrics. 2006; 20 (1): 105-108
in English | IMEMR | ID: emr-75664

ABSTRACT

This work was carried out to study the prevalence of Helicobacter pylon [H-P] infection in malnourished children and to prove the association -if any- between H-P infection and protein losing enteropathy [PLE] in these children. Stool samples of 100 children ranging in age from 3 to 24 months were studied. Those children included 40 under weight children, 40 marasmic children and 20 age and sex matched well nourished children as a control group. Stools from malnourished and control children were tested for H-P antigen and focal concentration of alpha 1- antitrypsin [FA-AT] using ELISA technique. Measurement of both parameters was repeated in H-P positive children 2 weeks after eradication therapy of H-P infection. The results proved that the prevalence of H-P infection amounted to 53% of malnourished children compared to 20% of control group. The mean values of FA-AT were significantly higher in malnourished children with positive H-P infection than those with negative infection [P <0.0001]. Following the eradication therapy of H-P infection, the mean values of FA-AT showed a significant decrease [P < 0.0001]. However, these values were still higher than those of the control group [P <0.0001]. H-P infection is an important co-factor in the etiology of some aspects of protein energy malnutrition [PEM]. H-P infection plays also a leading role in the pathogenesis of PLE and its therapeutic eradication can reverse some of these findings


Subject(s)
Humans , Male , Female , Helicobacter Infections , Helicobacter pylori , Protein-Losing Enteropathies/physiopathology , alpha 1-Antitrypsin , Feces , Enzyme-Linked Immunosorbent Assay , Prevalence
9.
Egyptian Journal of Hospital Medicine [The]. 2005; 21 (December): 82-94
in English | IMEMR | ID: emr-200689

ABSTRACT

The present study was performed to compare safety , efficacy of Proseal Laryngeal Mask Airway [PLMA], classic Laryngeal mask airway [LMA] and cuffed Endo Tracheal Tube [ETT] as a ventilatory device during controlled positive pressure ventilation and airway management , Haemodynamic response to insertion and removal, gastric tube insertion through either device, air leak detection and assessment of position by fiberoptic bronchoscope . Forty five ASA I or II patients aged between 18-55 years old , were divided equally into three groups of fifteen patients each , and airway management either through PLMA[groupI],classic LMA [groupII]and ETT [group III] . All patients were premedicated by zantac hydrochloride 150 mg orally at mid night and two hours before the operation – Anaesthesia was induced with fentanyl 2 ug/kg and propofol 2.5 mg /kg and maintenance was with a mixture of 50% N2O , 50% O2 and isoflurane 1 - 1.5 % and rocuronium 0.5 mg /kg followed by continous infusion of rocuronium 0.3-0.6 mg/kg/hr A proper size PLMA , classic LMA or ETT was selected oxygenation and ventilation were optimal in 100% in group I and III while in group II 80% optimal and suboptimal in 13.3% and failed in 6.7 % . Haemodynamic parameters showed that significantly increase in HR and MAP in the three studied groups especially at insertion and removal of the airway device with statisticaly significant difference between group I,II in comparison to group III, comparison of gastric tube insertion showed that positive insertion was 86.7% in group I and in 46.7% in group II, while in group III positive insertion was 100% air leak was detected by epigastric auscultation which signified lower leakage in PLMA group than LMA group . Position assessment by fiberoptic bronchoscope in PLMA group was grade 4 in 5 patients , grade 3 in 5 patients , grade2 in 4 patients and grade 1 in 1 patient while in LMA group it was grade 4 in 7 patients , grade 3 in 6 patients , grade 2 in 2 patients and grade 1 in no patient In conclusion :- PLMA and classic LMA could be better choices as ventilatory device in hypertensive and coronary artery disease patients

10.
Bulletin of Alexandria Faculty of Medicine. 2005; 41 (3): 547-555
in English | IMEMR | ID: emr-70174

ABSTRACT

The present work aimed at evaluating the usefulness of plasma elastase, D-dimer and fibrin [ogen] degradation products [FDPs] in comparison with doppler ultrasonography for the diagnosis of deep vein thrombosis [DVT] among 20 adult patients who were admitted to the emergency unit of Alexandria main University hospital with symptoms and signs suggestive of DVT. 10 healthy age and sex matched controls were also enrolled in this study. Plasma elastase, D-dimer were estimated by ELISA technique while FDPs by semiquantitative latex agglutination. They were done at presentation and 10 days after heparin therapy while they performed once in control group. It was found that the mean values of plasma D-dimer elastase and FDPs were significantly higher than those in control group both before [P[1]< 0.0001] and after therapy [P[2]< 0.0001] respectively. Post heparin therapy, the mean values of same parameters were significantly decreased when compared with pretreatment mean values using paired t-test [p= 0.000] for D dimer, elastase and FDPs respectively. As regards the extent of thrombus diagnosed by doppler ultrasonography, there was a significant increase in the mean values of D-dimer in proximal and all DVT than those in distal DVT while there was no significant difference between plasma elastase and FDPs and the extent of thrombus. A significant positive correlation was found between plasma D-dimer and elastase both before [r=0.845, p=/< 0.0001] and after heparin therapy [r= 0.764, p= 0.000]. No significant correlation was found between FDPs and the previous two parameters neither before nor after treatment. Doppler ultrasonography is a definitive test for diagnosis and localization of thrombus in DVT. Plasma D-dimer, elastase and FDPs can be used for initial evaluation of DVT suggestive patients in the emergency unit and further studies are needed to clarify their role in predicting the recurrence of DVT


Subject(s)
Humans , Male , Female , Ultrasonography, Doppler , Leukocyte Elastase , Fibrin Fibrinogen Degradation Products
SELECTION OF CITATIONS
SEARCH DETAIL