ABSTRACT
Genetic polymorphisms of encoding antigen B2 gene (AgB2) in Echinococcus granulosus were studied using PCR-RFLP and DNA sequencing among 20 Egyptian isolates. Five isolates from different host origins (humans, camels, pigs, and sheep) were collected and used. All examined isolates of each host group gave very similar patterns of PCR-RFLP after restriction enzyme digestion with AluI, with the gene size of approximately 140 bp and 240 bp for sheep and human isolates, and approximately 150 bp and 250 bp for pig and camel isolates. No digestion pattern was obtained after incubation of all studied isolates with EcoRI. These results reveal high intra-group homogeneity. DNA sequence analysis highlighted that human infecting strain showed 100% identity with respect to sheep infecting isolate, 96% and 99% with pig and camel infecting isolates, respectively.
Subject(s)
Animals , Humans , Camelus , Cysts/parasitology , Echinococcosis/parasitology , Echinococcus granulosus/genetics , Genetic Variation , Lipoproteins/genetics , Parasitic Diseases, Animal/parasitology , SheepABSTRACT
In the present study, micronuclei [MN] were determined in different sites in the oral cavity of 4 XP patients in order to determine whether a relationship exists between exposure to ultraviolet light and the level of chromosomal breakage. These sites were the right and left buccal mucosa, the dorsal tip of the tongue and the palate. Six controls matched for age and sex were examined concurrently. Beta-carotene were given to the four patients in a dose of 180 mg/week for 3 months. They were followed up every four weeks by taking buccal smear samples from the same sites order to detect any change in the frequency of MN. Before treatment, an unequal distribution of the frequency of MJN was found in the different samples sites of the oral cavity in XP patients with the greatest elevation in frequencies among cells collected from the tip of the tongue [0.76 +/- 0.15]. In constant, the MN frequency did not vary in samples from different sites obtained from the controls. The frequencies of MN were higher in XP patients than in controls for all sites expect the palate where technical difficulties were encountered