HLA-antigen association with cancer cervix: prognostic and genetic values

The present study was carried out on 26 patients with cancer cervix of various grades and histological types to investigate the possible association of HLA-A, HLA-B and HLA-DR in sera and paraffin embedded sections prepared from tumor tissue. Significant increase in frequency of HLA-B7, B14 and HLA-DR7 was detected in patient's sera compared to those of controls. HLA-DR antigen was expressed in tumor tissue in majority of patients. The intensity of immune expression varied inversely with the survival of patients and varied inversely with the degree of tumor differentiation and proportionally with the severity of associating immuno-competent cellular infiltrate. Despite the relatively little number of studied patient our data confirms an immuno-genetic base in susceptibility to the disease and points to HLA-antigen expression in tumor tissue as a negatively influencing factor, not to be forgotten during evaluating the prognosis of patients with cancer cervix

Toxicity of methotrexate on fertility and internal organs of male albino rats

Fifty male adult albino rats with average weight of 220 - 250 gm were used in this study. Animals were divided into four test groups and a control group each comprised of ten rats. Test animals received methotrexate [MTX] in a therapeutic constant low dose of 7.5 mg/kg/ week for eight weeks. The effects of the drug were assessed by histopathological, biochemical and spermatozoal examination immediately after its stoppage and within one month recovery period. Significant histopathological changes in internal organs of albino rats associated with marked functional biochemical abnormalities were observed. Also there was marked alteration in sperm count, motility and increased percentage of abnormal forms. Gradual improvement was observed during the recovery period

Cytopathological significance of eosinophilic pleural effusion

388 cases of pleural effusions were examined and out of them 21 eosinophilic effusions were detected. Malignant lesions were present in 9 out of the all 21 detected cases constituted [42.85%] while, non neoplastic lesion were present in the remaining 12 cases. These were heart failure [4 cases], bronchial asthma [one case] SLE [one case] and 6 cases were idiopathic

Evaluation of nucleolar organizer regions in Non-hodgkin's Lymphoma

46 lymph nodes specimens of N.H.L were included in the present study. Paraffin processed sections were prepared and stained with the following: 1. Hx. and E. for histopathological typing of N.H.L. according to the working formulation classification. 2-Argyrophil silver staining for analysis of NORs. Our results demonstrated there is well defined difference: between low, intermediate and 5 of grade lymphomas on the basis of NORs count of their cells.Also, NORs count increased in patient living than 2 years. Our findings documented the considerable value of the Ag-NORs for both grading and prognosis in NHL

Autograft ossicles in cholesteatomatous ears: a clinicopathological study

The results of this study revealed that squamous epithelium was never found in direct contact or in the deeper vascular spaces, In almost all cases it was separated from the bone by granulation tissue. Peneteration of cholesteatoma into the deep vascular spaces of bony ossicles was never seen in our specimens. The examined ossicles after autoclaving showed no vital epithelial rests and were bacteriologicaly sterile. Our histopathological and bacteriological findings have encouraged us to consider reimplation of autogenous drilled autoclaved ossicles as nearly safe and risk-free process

Characterization of nucleolar organizer regions [NORs] in benign and malignant epithelial breast lesions

This study was conducted on 36 benign and 36 malignant epithelial lesions.They were processed as paraffin sections and attained with: 1] Hx. and E. for : a-Histopathological typing of the different lesions b- Histopathological grading of the malignant lesions 2] Argyrophil silver colloid technique for NORs demonstration It has been shown that, in the present study, NORs can differentiate between benign and malignant lesions, a finding which facilitate the resolution of some well-known diagnostic problems in the breast. The rapidity of the method of staining, the ease of interpretation and being inexspensive add advantages to it. Moreover, NORs number in malignant lesions can provide an information about the proliferative status of the cells and so it can be used as a prognostic factor supplementary to other prognostic indices in breast carcinoma