ABSTRACT
Objective: To establish a RP-HPLC-DAD method for simultaneous determination of alisol C, alisol F, alisol C 23-acetate, alisol L, alisol F 24-acetate, alisol A, alisol A 24-acetate, alisol G, alisol B, alisol B 23-acetate, and 11-deoxy-alisol B in Alismatis Rhizoma. The content of 11 components from 25 batches of samples collected from different habitats was determined and compared by means of this established method. Methods: The separation was achieved on a Ultimate XB-C18 column (150 mm × 4.6 mm, 5 μm) with the mobile phase consisting of water-acetonitrile to be gradient elution. Flow rate was 1 mL/min and column temperature was 30℃. The optimum detection wavelengths were 208 and 245 nm. Results: The linear ranges of alisol C, alisol F, alisol C 23-acetate, alisol L, alisol F 24-acetate, alisol A, alisol A 24-acetate, alisol G, alisol B, alisol B 23-acetate, and 11-deoxy-alisol B were 0.313-17.210 (r = 0.999 9), 0.504-11.880 (r = 0.999 9), 0.284-9.369 (r = 0.999 9), 0.146-2.680 (r = 0.999 9), 0.254-5.596 (r = 0.999 8), 2.500-66.000 (r = 0.999 8), 1.620-35.640 (r = 0.999 9), 1.575-20.475 (r = 0.999 9), 1.525-57.268 (r = 0.999 6), 3.035-118.362 (r = 0.999 9), and 0.816-16.880 μg/mL (r = 0.999 8), respectively. The average recoveries (n = 6) were 98.76%, 100.24%, 97.97%, 100.14%, 99.88%, 96.54%, 97.27%, 98.85%, 99.45%, 98.85%, and 98.13% respectively. Conclusion: The RP-HPLC-DAD method is feasible and accurate, which could provides the scientific evidence for quality control of Alismatis Rhizoma.
ABSTRACT
OBJECTIVE: To develop a simultaneous quantitative analysis method of four triterpenoids in Alismatis Rhizoma by multi-components by single maker (QAMS). METHODS: Four main triterpenoids (alisol A, alisol A 24-acetate, alisol B and alisol B 23-acetate) were selected as analytes to evaluate the quality of Alismatis Rhizoma. Alisol B 23-acetate was used as the internal reference standard and three relative correction factors (RCFs) to alisol B 23-acetate were calculated. These RCFs were obtained by high performance liquid chromatography coupled with diode array detector under various chromatographic conditions. Then 31 batches of Alismatis Rhizoma samples collected from different areas were determined by both external standard method (ESM) and QAMS. RESULTS: The RCFs were obtained with good reproducibility (RSD≤2.25%). No obvious differences were observed between the analysis results of QAMS method and ESM method (RSD≤3.52%). CONCLUSION: The established QAMS method is reliable and feasible for simultaneous analysis of four triterpenoids and could be used for quality control of Alismatis Rhizoma.