ABSTRACT
@# Objective: To explore the molecular mechanism of miR-17-5p regulating the proliferation and invasion of nasopharyngeal carcinoma cells by regulating the expression of breast cancer metastasis suppressor 1 like (BRMS1-like or BRMS1L) gene. Methods:A total of 40 cases of nasopharyngeal carcinoma tissues and corresponding paracancerous tissues resected from nasopharyngeal carcinoma patients, who were admitted to the General Hospital of Pingdingshan Shenma Medical Group during January 2014 to December 2017, were included in this study; in addition, nasopharyngeal carcinoma cell lines CNE 2, HONE 1, C666-1 and nasopharyngeal immortalized epithelial cell line NP69 were also collected for this study. The expression of miR-17-5p in nasopharyngeal carcinoma tissues and cell lines was detected by qPCR. The targeted relationship between BRMS1L and miR-17-5p was predicted by the StarBase and verified by the Dual luciferase reporter gene assay. Effects of transfection of miR-17-5p mimics and inhibitors on the expression of BRMS1Lin CNE2 cells were detected by WB assay. CCK-8, Transwell and Flow cytometry were used to detect the effects of miR-17-5p/BRMS1L axis on the proliferation, migration, invasion and apoptosis of CNE 2 cells. Results: miR-17-5p was highly expressed in nasopharyngeal carcinoma tissues and cell lines (P<0.05 or P<0.01). Knockdown of miR-17-5p significantly inhibited proliferation, invasion and migration of CNE2 cells but promoted apoptosis (P<0.05 or P<0.01); miR-17-5p targeted BRMS1Land down-regulated its expression. Over-expression of BRMS1Lsignificantly inhibited the proliferation, invasion and migration of CNE2 cells but promoted apoptosis (all P<0.01); while simultaneous over-expression of miR-17-5p and BRMS1L reversed the above effects (all P<0.01). Conclusion: miR-17-5p promoted proliferation, invasion, migration and inhibited apoptosis of CNE 2 cells by down-regulating the expression of BRMS1L.
ABSTRACT
To conduct a Meta analysis with 12 related published studies to compare the effect of operative and conventional treatment for acute ankle sprain. A systematic research on the published paper was conducted for randomized controlled trial studies. Three data bases were searched, including Pubmed, EMbase and ISI web of Knowledge, and we compared the efficacy of two treatment methods, operative treatment and functional treatment, for acute ruptures of lateral ankle. A total of 15 related RCT studies were included in our study. RevMan software was taken to analyze the data. The 15 studies involved 1413 mostly young adults, 665 of them received surgical treatment, while 748 received conventional treatment. The results showed surgical treatment is more beneficial as compared to conventional treatment in terms of ankle activity and instability, with the OR[95%CI] of 5.15 [1.06-25.03] and 1.72 [1.31-2.26], respectively. However, the surgical treatment was accompanied with great risk of complication, including DVT, tenderness of scar, sensory loss and wound infection or necrosis. Surgical treatment is better than the conventional treatment alone, especially for the outcome of the ankle activity and instability. Further high quality randomized controlled trials are required.