Study on map analysis and trend prospect of campus thermal environment based on CiteSpace / 中国学校卫生

Objective@#To grasp the research dynamics and development trend of the thermal environment of campus buildings in China, so as to lay the foundation for in depth research and provide a reference basis.@*Methods@#Based on 479 documents in CNKI from 2000 to 2020, the documents were visualized and analyzed by keywords, including co occurrence, clustering, outbreak and author cooperation network in CiteSpace.@*Results@#In the past 20 years, the research on thermal environment of campus buildings in China had gone through a period of formation development growth, and was at a high growth stage now. The word frequency of "thermal comfort, thermal environment, indoor thermal environment, natural ventilation" was greater than 50, which was a high frequency keyword. The highest outbreak rate of "green campus" was 3.75, and "university library, university building, microclimate and green building" was in the outbreak period. And LIU Jiaping, LIU Zehua, WANG Hongguang and others were highly productive authors and have formed cooperative network groups with their own cores, but the cooperation among the network groups was less to be strengthened.@*Conclusion@#The research dynamics of the thermal environment of campus buildings is closely related to the policy development in China, and the research on "green campus, campus energy saving optimization, and university buildings" based on human thermal comfort theory is a hot topic of continuous attention.

TNF-alpha increases angiogenic potential in a co-culture system of dental pulp cells and endothelial cells

Abstract We recently demonstrated that a co-culture system of human umbilical vein endothelial cells (HUVECs) and human dental pulp stem cells (hDPSCs) could enhance angiogenesis ability in vitro. However, whether tumor necrosis factor α (TNF-α) could promote blood vessel formation during pulp regeneration remained unknown. The aim of this study was to investigate the effects of TNF-α on the formation of endothelial tubules and vascular networks in a co-culture system of hDPSCs and HUVECs. hDPSCs were co-cultured with HUVECs at a ratio of 1:5. The Matrigel assay was performed to detect the total tubule branching lengths and numbers of branches, and the Cell-Counting Kit 8 assay was performed to examine the effect of TNF-α on cell proliferation. Real-time polymerase chain reactions and western blot were used to detect vascular endothelial growth factor (VEGF) mRNA and protein expression. The Matrigel assay showed significantly greater total branching lengths and numbers of branches formed in the experimental groups treated with different concentrations of TNF-α compared with the control group. The decomposition times of the tubule structures were also significantly prolonged (P < 0.05). Treatment with 50 ng/ml TNF-α did not significantly change the proliferation of co-cultured cells, but it significantly increased the VEGF mRNA and protein expression levels (p < 0.05). In addition, the migration abilities of HUVECs and hDPSCs increased after co-culture with TNF-α (p < 0.05). TNF-α enhanced angiogenic ability in vitro in the co-culture system of hDPSCs and HUVECs.