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ObjectiveTo study the expression of AKT2 gene in liver cancer and its relationship to tumor progression.MethodsThe expression of AKT2 in liver cancer was detected by SP immunohistochemical stainin and reverse transcription polymerase chain reaction (RT-PCR).Four patients with benign liver tumors were used as control.ResultsThe positive rates of AKT2 in liver cancer tissue and benign control tissue were 62.5％ (28/32) and 0％ (0/4),respectively.The difference was significant.In addition,a positive expression of AKT2 correlated significantly with poor differentiation,positive lymph node and distant metastasis.The median survival after surgery was significantly shorter in patients with positive than with negative AKT2 (76d vs 463d).ConclusionThe detection of AKT2 was useful in assessing the progression of liver cancer,in determining prognosis and eventually in rendering a possible target for novel therapeutic strategies.
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Objective To investigate the clinical application value of laparoscopy-assisted subtotal colectomy with antiperistalsis cecorectal anastomosis for slow transit constipation (STC).Methods From September 2007 to October 2010,a total of 31 patients with STC underwent laparoscopic-assisted subtotal colectomy with antiperistalsis cecorectal anastomosis.A follow-up survey was completed at 3 and 12 months after the operation.Results No death or conversions to open operation occurred.The mean operation time,mean intraoperative blood loss and mean post-operative hospitalization were 260 min (180-310 min),60 ml (30-120 ml) and 8d (6-11 d),respectively.No postoperative infection,anastomotic stoma,adhesive intestinal obstruction or other perioperative complications occurred.In 3-month follow-up,constipation was significantly alleviated in 23 patients,mild diarrhea occurred in 5,diarrhea in 2 and mild recurrence in 1.Constipation was significantly alleviated in 25 patients,mild diarrhea occurred in 5 and mild recurrence in 1 at 12-month follow-up.Conclusion Laparoscopy-assisted subtotal colectomy with antiperistalsis cecorectal anastomosis is safe,effective and less invasive for STC.
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Objective To study the effects of inhibing AKT2 by siRNA on SMMC7721 liver cancer cells proliferation,apoptosis,migration and invasion.Methods The siRNA targeting AKT2 was designedandthe SMMC7721AKT2- siRNAplasmidwasconstructed andtransfected into SMMC7721 cells.The stable cell lines were screened by G418.The effects of AKT2 by siRNA on SMMC7721 liver cancer cells,growth inhibition was evaluated by MTT assay.Cell cycle was analyzed by flowcytometry (FCM).Protein of P27 and CyclinD1 was evaluated by Western-blot.The ability of migration and invasion was evaluated by wound healing and Transwell assay.ResultsThe growth of SMMC7721 cells was significantly inhibited by siRNA (P＜0.05).Flow cytometry display that AKT2 by siRNA can induce G1 phase arrest,the ratio of G1 phase increased homologously and S phase declined homologously.The protein of CyclinD1 was declined and the protein of P27 was increased by Western-blot.Wound healing and Transwell assay show that the ability of cells,migration and invasion was inhibited by AKT2 by siRNA.Conclusion AKT2 by siRNA can significantly inhibit the growth of SMMC7721 cells,arrest cell cycle.AKT2 by siRNA can inhibit the ability of invasion and migration of SMMC7721 cells.
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Objective To evaluate the efficacy of endoscopic thyroidectomy via the breast areola. Methods From August 2004 to May 2008,36 patients with benign thyroid nodules (
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Objective To investigate causes and the treatment of jaundice following laparoscopic cholecystectomy (LC). Methods A retrospective analysis of records of 11 cases of jaundice following LC out of 2047 cases between October 1995 and December 2001 was made. Results The incidence of postoperative jaundice was 0.54% (11 of 2047). Jaundice was due to bile leakage (45.4%, 5 of 11), residual choledocholithiasis (18.2%, 2 of 11), acute pancreatitis (18.2%, 2 of 11), biliary stricture (9.1%, 1 of 11) and acute liver infraction (9.1%, 1 of 11). Three kinds of procedures-endoscopic operation, open operation and conservative treatment-were executed respectively and had obtained satisfactory results. Conclusions Bile leakage and residual choledocholithiasis were the leading causes of jaundice following LC. Prompt etiologic therapy may effectively relieve the symptoms.