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Objective To compare the effects of the different contrast media concentration on the visualization of pancreatic arteries using 64-Detector Spiral CT. Methods 120 cases who underwent abdominal enhancement CT scanning were randomly divided into group A (n = 60) and B (n = 60). The contrast media concentration was 300 mgI/ml for group A and 370 mgI/ml for group B respectively, both groups shared the same contrast media dose algorithm and injection speed, with 0.5 gI/kg body weight and 4 ml/s respectively. Reformating the pancreatic arteries via MIP (maximum intensity projection), VR (volume rendering) and MPR (multi-planar reconstruction). Results The CT value of the abdominal aortic celiac opening of group B is significantly higher than A (P < 0.01). The visualization scores of pancreatic arteriesin Group B are significantly higher than A (P < 0.05). The visualization ratios of pancreatic arteriesin Group Bare higher than A, the differences of AIPDA、PIPDA、DPA、TPA、PMA、CPA between the 2 Groups have statistically significant (P < 0.05). Conclusion Pancreatic arteries could have a better visualization by using 370 mgI/ml contrast media in pancreatic artery scanning.
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Moutan Cortex and Paeonia Radix are also traditional Chinese herbal medicines. The market is in great demand. However, the market sales of Moutan Cortex and Paeonia Radix have problems such as pesticide residues, excessive heavy metals and degraded quality, which affect their safety and effectiveness. The establishment of a pollution-free and planting system can effectively reduce the pesticide residue and heavy metal content and improve the quality of the medicinal materials while ensuring the production of Moutan Cortex and Paeonia Radix. The article uses field data which based on P.suffruticosa and P.lactiflora cultivation, combined with growth habits and distribution of origin, this research has developed a pollution-free and planting technology system for P.suffruticosa and P.lactiflora. The system includes planting base regionalization, field management, fertilization, pest control and so on, which provides reference for P.suffruticosa and P.lactiflora.
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Rabdosia (Bl.) Hassk is a medicinal plant rich in enantiomers such as 15α-acetoxyl-6, 11α-epoxy-6α-hydroxy-20-oxo-6, 7-seco-ent-kaur-16-en-1, 7-olide and 15α-dihydroxy-6β-methoxy-6, 7-seco-6, 20-epoxy-1, 7-olide-ent-kaur-16-ene. It has high medicinal value and huge market potential, but the yield from wild Rabdosia (Bl.) Hassk species cannot meet the needs of marketization. Carrying out pollution-free cultivation of Rabdosia rubescens (Hemsl.) Hara and Rabdosia serra (Maxim.) Hara has become the most direct and effective measure to solve this problem in order to ensure the sustainable use of the resources of Rabdosia. This paper summarized a set of non- pollution cultivation system of R. rubescens and R. serra to achieve the"safe, effective, stable and controllable"target.
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Objective To explore the risk factors in post-polypectomy hemorrhage in rectum and to discuss the appropriate interventions.Methods A total of 313 patients with 373 polypi were included in this study. The clinical data were analyzed by SPSS 16 software. Results There were 313 patients with colorectal polypus curatively resected and 373 polypi in total.There were 11 (3.5％) patients subjected to post-polypectomy hemorrhage in rectum.Regression analysis showed that the independent risk factor of postpolypectomy hemorrhage in rectum was the hypertension of patients (P ＜ 0.01 ) and this hemorrhage had no significant correlations with patientg'ender,age,size of polypus,pathological characteristics and the methods of polypectomy.Conclusions Hypertension of patients is an independent risk factor in post-polypectomy hemorrhage.
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A derivatization method for the determination of oligosaccharides with high sensitivity by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry(MALDI-TOF-MS) based on a labeling regent 1-(4-Cyanophenyl)-4-piperidinecarbohydrazide(CPH) was proposed. The hydrazones were generated by the reaction of the reducing ends of oligosaccharides and the hydrazide of CPH. The derivatives were directly analyzed by MALDI-TOF-MS. Reaction conditions such as reaction temperature, concentration of acetic acid and excess ratio of CPH regent were optimized. When the oligosaccharides reacted with 100 time excess of CPH at 100 ℃ and in 0.125% acetic acid (V/V), the yield was highest. The results showed the derivatization with CPH increased the sensitivity of MS detection and this method was suitable for the analysis of oligosaccharides′ compositions.
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Objective To explore the treatment of complications associated with non-functioning renal allografts using embolism of renal artery.Methods Six patients with non-functioning renal allografts were treated with renal artery embolism,morphous,blood flow of allografts and symptoms were observed.Results Hematuria disappeared in 3 days after embolism;blood pressure levels controlled by drugs were in a normal region.Using color Doppler sonography,we found no blood flow in 4 cases,discontinuous blood flow in 2 cases.Blood flow disappeared in all 6 allografts 3 months later,and the allografts had atrophied.Conclusion Embolism of renal artery may be an effective and safe method for the treatment of complications associated with non-functioning renal allografts,especially for some severe patients;it could avoid the risks of operation.
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Objective To study the quantitative method of determination of Fas ligand (FasL) and T-cell intracellular antigen-1 (TIA-1) in renal allografts and to evaluate its clinical significance. Methods The magnitude of intragraft FasL and TIA-1 mRNA was quantified by competitive PCR in 42 samples obtained to diagnose the etiology of graft dysfunction, and the results were expressed as the ratio of picograms of target gene to picograms of ?-actin, and were compared with the histopathogical diagnosis based on Banff criteria. Results Of all 42 samples, the transcripts were detectable in 11 for FasL and 27 for TIA-1. Intragraft expression of FasL and TIA-1 was significantly up-regulated in acute rejection group compared with chronic rejection group (P
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Objective To define the relationship between genes expression of 5 immune effector molecules and acute rejection of renal allografts. Methods The magnitude of intragraft Fas, Fas ligand (FasL), granzyme B(GB) , perforin(P) and T cell intracellular antigen-1 (TIA-1) mRNA were quantified by competitive PCR in 42 samples. Results were expressed as the ratio of picograms of target gene to picograms of ?-actin, and were compared with the histological diagnosis based upon Banff criteria. Results Of all 42 tissues, the transcripts were detectable in 41 for Fas, 11 for FasL, 19 for GB, 33 for P and 27 for TIA-1. Intragraft expression of FasL, GB, P and TIA-1 but not Fas was significantly elevated in acute rejection group as compared with chronic rejection group ( P
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Objective:To construct a mutispecific internal control for competitive RT-PCR analysis of Fas、FasL、GB、P、TIA-1 and ?-actin.Methods:Invitro synthesized fragments were amplified by PCR,there are two products,of which 145 and 147 bp.The 145 bp one contained 5′ primer sequences of Fas、FasL、GB、P、TIA-1 and ?-actin,another contained 3′ primer sequences of the same genes.The products with restriction sites were inserted into the vector PKF_3.Results:Restriction enzyme analysis and DNA sequencing were used to identify the recombinant plasmid,corresponding internal control was obtained by the amplification of the recombinant plasmid with each primer pair.The mutispecific internal control was then used for quantitative detection of TIA-1 in peripheral blood leukocytes from a patient with acutely rejecting allograft.Our study on Fas、FasL、GB、P、TIA-1 and ?-actin showed that the coamplified templates accumulated in a parallel manner throughout not only the exponential phase.Conclusion:The mutispecific internal control can be used for quantitative detection of the six genes. [