ABSTRACT
Method development for the determination of major constituents from Khamin-chan, Curcuma longa Linn., which are curcumin (CI), demethoxycurcumin (CII) and bisdemethoxycurcumin (CIII) by high performance liquid chromatography (HPLC) has been developed. The reverse phase HPLC system consisted of a Dupont Zorbax ODS column, and 36% tetrahydrofuran (THF) in 10% glacial acetic acid as a mobile phase. The column effluent was monitored by a Jasco FP-210 Spectrofluorometer detector with Ex = 420 nm, and Em = 530 nm. The correlation of calibration curve of CI, CII and CIII were excellent with their r’s value 0.99 in all cases. The reproducibility study gave a very good results with the percent C.V. of about 5 percent or less for all three curcuminoids. With this analytical procedure, pharmacokinetic study of Khamin-chan in in situ model was undertaken. It was found that all three curcuminoids were poorly absorbed. About 60-70 percent of curcumins were absorbed within 30 minutes. While there was no statistical difference in the absorption rate between CI and CII, the poorest absorption compound was found to be CIII.
ABSTRACT
The relationship of theophylline concentration in serum and saliva was studied in 10 Thai asthmatic children receiving “Elixir Quibron” and Tablet Aminophylline orally in a cross over design. There was an excellent linear relationship between theophylline concentration in serum and saliva regardless of dosage forms. Proportionality factor that relates saliva to serum concentration obtained in this study was 1.60. Serum Theophylline concentration can be estimated by analysis of saliva concentration multiplied by 1.60. These observations suggest that determination of theophylline concentration in saliva is an effective, convenient, noninvasive, and painless method for routine indirect monitoring of serum theophylline concentration in Thai asthmatic children.