ABSTRACT
Objective: To study the chemical constituents from Sabia parviflora. Methods: Various column chromatographic techniques were used to separate and purify the chemical constituents which structures were elucidated by spectral analysis. Results: Fifteen compounds were isolated and identified as 1,3,5-trimethoxybenzene (1), vanillic aldehyde 4-O-β-D-glucopyranoside (2), 2,6-dimethoxy-4-hydroxybenzyl alcohol 1-O-β-D-glucopyranoside (3), rel-5-(3S,8S-dihydroxy-1R,5S-dimethyl-7-oxa-6-oxobicyclo [3,2,1]-oct-8-yl)-3-methyl-2Z,4E-penta-dienoic acid (4), protocatechuic acid (5), 4-quinolinone-2-caboxylic acid (6), 3,4,5-trimethoxy benzoic acid (7), pyrocatechol (8), syringic acid-4-O-β-D-glucopyranoside (9), (-)-(7α,8S)-erythro-1-C-syringylglycerol 4-O-β-D- glucopyranoside (10), 3,4,5-trimethoxy benzoic acid (11), 1,2,4-trihydroxybenzene (12), ferulic acid (13), vanillic acid (14), and p-hydroxybenzoic acid (15). Conclusion: Compounds 1-11 are isolated from the genus of Sabia for the first time, and compounds 12-15 are isolated from this plant for the first time.
ABSTRACT
ObjeCtive To investigate the value of protein biochip in the diagnosis of Lyme borreliosis. Methods The serum IgM and IgG antibodies against flagellin were detected by flagellin coated-and N-hydroxysuccinimide (NHS) modified-biochips in 82 patients with neuroborreliosis (NB),35 patients with erythema migrans (EM) and 44 normal controls.The results were compared with those from enzyme-linked immunosorbent assays (ELISA) and Western blot.Results The levels of both IgM and IgG antibodies were significantly higher in the NB patients than those in the normal controls (P