ABSTRACT
<p><b>OBJECTIVE</b>To establish a nude mouse model of nasopharyngeal carcinoma (NPC) lymph node metastasis and screen the signature genes associated with the metastasis.</p><p><b>METHODS</b>The NPC 5-8F-EGFP cells were inoculated into nude mice, from which a 5-8F-LN cell line with lymph node metastasis potential was obtained. The lymphatic metastasis-related signature genes of breast cancer and head and neck squamous cell carcinoma were screened by data mining method.</p><p><b>RESULTS</b>The NPC cell lines 5-8F and 6-10B showed 307 differentially expressed genes by microarray analysis, from which 20 overlapping genes were identified, and 3 overexpressed genes were found with probable metastasis potential, namely the ADM, IRF1, and CAV1 genes. Quantitative RT-PCR validated the data mining results in the 5-8F-EGFP, 6-10B-EGFP, NP69, and 5-8F-LN cell lines. The 3 NPC cell lines 5-8F-EGFP, 6-10B-EGFP and 5-8F-LN showed significantly higher expressions of IRF1 than NP69 cells (P=0.008, 0.022, and 0.006, respectively. The expression level of CAV1 in 5-8F-EGFP cells was significantly higher than that in 6-10B-EGFP cells (P=0.014), but ADM expression showed no significant difference between the 4 cell lines.</p><p><b>CONCLUSIONS</b>IRF1 may play an important role in the progression of NPC. The overexpression of CAV1 in 5-8F-EGFP cells can be associated with the high metastatic potential of the cells.</p>
Subject(s)
Animals , Humans , Mice , Adrenomedullin , Genetics , Caveolin 1 , Genetics , Cell Line, Tumor , Disease Models, Animal , Gene Expression Profiling , Interferon Regulatory Factor-1 , Genetics , Lymphatic Metastasis , Mice, Inbred BALB C , Mice, Nude , Nasopharyngeal Neoplasms , Genetics , Pathology , Neoplasm Transplantation , Reverse Transcriptase Polymerase Chain Reaction , Transplantation, HeterologousABSTRACT
<p><b>OBJECTIVE</b>To investigate cyclooxygenase-2 (COX-2) expression of in colorectal carcinoma cell lines and tissues and its clinical implications.</p><p><b>METHODS</b>SP immunohistochemistry was used to detect COX-2 protein in SW480 and SW620 cell lines and 50 primary colorectal carcinoma and 50 lymphoma metastasis carcinoma specimens. Real-time PCR was used to detect COX-2 mRNA expression in SW480 and SW620 cell lines.</p><p><b>RESULTS</b>SW480 and SW620 cell lines both highly expressed COX-2. The positive expression levels of COX-2 increased significantly in lymph node metastatic carcinoma in comparison with primary colorectal carcinoma (P<0.05). The expression COX-2 mRNA in SW620 cell line was higher than that of SW480 cell line, showing a mean expression increment of 2.268 folds in SW620 cell line relative to SW480.</p><p><b>CONCLUSION</b>Elevated COX-2 expression may be associated with lymph node metastases of colorectal carcinoma.</p>