ABSTRACT
When this paper was first published the following ethical statement was omitted in error: This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of Tianjin Institute of Pharmaceutical Research. The authors would like to apologise for any inconvenience caused.
ABSTRACT
When this paper was first published the following ethical statement was omitted in error: This study was approved by the Ethics Committee of AAALAC (NO. 001488) and carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of Tianjin Institute of Pharmaceutical Research. The authors would like to apologise for any inconvenience caused. DOI of original article: https://doi.org/10.1016/j.chmed.2018.12.002
ABSTRACT
Objective: To identify the therapeutic effect and possible mechanisms of Chinese medicine Sanqi Tongshuan Tablets (SQTS) on sequelae post-stroke in rats. Methods: The rat cerebral ischemia sequelae post-stroke models were successfully induced by blocking the middle cerebral artery with electric coagulator after the seventh week and balance beam test ≤ 4. The rats were then received with SQTS (0.5, 1, and 2 g/kg) and Naodesheng (NDS, 1.24 g/kg), Vinpocetine (VP, 1.55 mg/kg) for 30 d. The beam-walking test and shuttle test were performed before and after 10, 20, and 30 d of administration. In addition, histopathology changes and GAP-43, GFAP were measured by H&E staining and immunohistochemisty. Results: The model displayed signs of brain damage on motor function, learning and memory function and histopathology. After 30 d of treatment, SQTS at different doses (0.5, 1.0, and 2.0 g/kg) restored the beam-walking scores by 21.7% (P > 0.05), 30.4% (P > 0.05), and 39.1% (P 0.05), 50.0% (P > 0.05), and 75.0% (P < 0.05), respectively. On the other hand, the histological changes were less severe and the GAP-43 expression increased in hippocampal CA1 and cortical region. Conclusion: SQTS showed therapeutic benefits on sequelae post-stroke in rats, which might be through the pathway of regeneration or neuroplasticity.
ABSTRACT
<p><b>AIM</b>To study the effects of 1-(2,6-dimethylphenoxy)-2-(3,4-dimethoxyphenylethylamino) propane hydrochloride(DDPH) on brain ischemia injury in rats.</p><p><b>METHODS</b>By using the middle cerebral artery occlusion (MCAO) induced by nylon surgical thread inserted through the internal carotid artery into the anterior cerebral artery in rats, the effects of DDPH on neuron defects(ND) and infarct size(IS) were investigated. Using incomplete cerebral ischemia in rats, the effects of DDPH on superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in brain tissue and pathological changes in rats were studied.</p><p><b>RESULTS</b>DDPH at the dose of 10 mg.kg-1 i.p. 30 min before ischemia decreased the ND 3 h after ischemia. The IS declined 24 h after ischemia as well. Meanwhile, DDPH was found to increase SOD activity and reduce the MDA content, as well as mitigate pathological damage, of neuron after brain ischemia in rats.</p><p><b>CONCLUSION</b>DDPH showed protective effects on brain ischemia, probably related to its properties of calcium antagonistic effect and increasing the activity of superoxide dismutases.</p>