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Journal of Southern Medical University ; (12): 1195-1198, 2014.
Article in Chinese | WPRIM | ID: wpr-312608

ABSTRACT

<p><b>OBJECTIVE</b>To study the association of methylenetetrahydrofolate reductase (MTHFR) gene C677T mutation and plasma homocysteine (Hcy) levels with hyperlipidemia.</p><p><b>METHODS</b>Blood samples were collected from 1591 adults for detecting MTHFR gene C677T polymorphism with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), plasma Hcy levels with enzymatic cycling method, and blood lipid levels as well. The patients were divided according to the lipid levels into hyperlipidemia group (n=694) and healthy control group (n=897) and the differences in MTHFR gene C677T polymorphisms and plasma Hcy levels were compared.</p><p><b>RESULTS</b>The hyperlipidemia group and healthy control group showed no significant differences in CC, CT, or TT genotype frequencies or C and T allele frequencies of MTHFR C677T gene, and had comparable plasma Hcy levels (P>0.05). Patients with 3 different MTHFR C677T genotypes had significant differences in plasma Hcy levels (P<0.01) but not in blood lipid levels (P>0.05). Pairwise comparison indicated a significantly higher plasma Hcy level in TT genotype than in CC and CT genotypes (P<0.01), and the latter two genotypes showed no significant difference (P>0.05).</p><p><b>CONCLUSION</b>MTHFR C677T polymorphisms and plasma Hcy levels are closely related but neither of them is associated with hyperlipidemia. The TT genotype is associated with a significantly higher plasma Hcy level than CC and CT genotypes.</p>


Subject(s)
Adult , Humans , Gene Frequency , Genotype , Homocysteine , Blood , Hyperlipidemias , Blood , Genetics , Methylenetetrahydrofolate Reductase (NADPH2) , Genetics , Mutation , Polymerase Chain Reaction , Polymorphism, Genetic , Polymorphism, Restriction Fragment Length
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