ABSTRACT
The function of physics courses is important for medical students. The physics courses not only teach them knowl-edge of physics, but also train them scientific literacy. Some problems and mistakes were present in the physics courses for medical students in China. In this paper some views are advanced to solve this problems or mistakes. The physics courses in medical colleges must retain its physical characteristic and should diversify in education method.
ABSTRACT
The changes in the cellular main components of the mouse erythroleukemia cell line MEL for TFAR19 gene transfection were studied by the technology of Fourier transform infrared spectroscopy (FTIR). Using the method of gene transfection with liposome, we obtained MEL-TF19 cell line, which stably carries TFAR19, a novel apoptosis-related gene. The expression of the gene on mRNA level was confirmed by RT-PCR. Then, FTIR spectra of the cells were measured in the course of apoptosis induced by serum deprivation. Our results indicated that after being transfected with TFAR19 gene, MEL-TF19 cells exhibited relatively higher protein content, higher transcriptional activity, and relatively lower phospholipid content as compared with those exhibited by MEL cells. All the above changes reflect the apoptosis-promoting effect of TFAR19 gene, and maybe account for the cellular rheological changes after TFAR19 gene transfection, which were discovered in our previous study.
Subject(s)
Animals , Mice , Apoptosis , Genetics , Apoptosis Regulatory Proteins , Cell Line, Tumor , Genes, Tumor Suppressor , Leukemia, Erythroblastic, Acute , Genetics , Pathology , Molecular Sequence Data , Neoplasm Proteins , Genetics , Pharmacology , Spectroscopy, Fourier Transform Infrared , TransfectionABSTRACT
Rabbit blood samples were exposed to constant magnetic field within a range of intensity from 0.05 T to 0.35 T for 10 to 50 minutes, the propersities of hemorheology were measured by ektacytometry. The following results were obtained: The blood viscosity was lowed;The RBCs deformability was not changed obviously except for 10 minutes of exposure when the RBC＇s deformability was strongly decreased; The orientation index was increased; The results indicate that the magnetic field increases the RBC surface charge and results in the decrease of blood viscosity.