ABSTRACT
Objective To establish a model of rat endotoxic pulpitis induced by Lipopolysaccharide (LPS);to study the dynamic expression and location of Oct-4B in rat pulpitis model at different time;To evaluate the role of Oct-4B on pulp injury and reparation in rat endotoxic pulpitis. Method The slides were made and the dynamic expression and location of Oct-4B was examined by immunohistochemistry. Results Oct-4B was moderately positive in the pulp fibroblasts and odontoblasts in 2 weeks. After 2 weeks, it was still positive in odontoblasts and fibroblasts. Up to 3 weeks, it was weakly positive in odontoblasts and fibroblasts. Conclusion Oct-4B is dynamically expressed in the rats with pulpitis, and may play an important role in the incidence,development and recovery of pulpitis.
ABSTRACT
<p><b>OBJECTIVE</b>To compare the hardness development of dual-cured self-adhesive and universal resin cement in simulated root canal.</p><p><b>METHODS</b>The light-proof half-cylinder steel slot with one end open were syringed and filled respectively by self-adhesive A (RelyX Unicem), B (BisCem) and universal C (DUOLINK) resin cements, then the open end of slot was irradiated directly by a light unit for 20 s. Specimens were stored in a light-proof box for 0.5 h, Knoop microhardness was measured along the vertical surfaces of specimens from 1 mm to 10mm depth at 1 mm intervals. The same measurements were taken at 24 h and 120 h after irradiation. Data were analyzed by One-way ANOVA.</p><p><b>RESULTS</b>Hardness of each group decreased with the increase of simulated canal depth (P<0.001), however hardness showed no significant change between 5 mm and more depth of group A, between 4 mm and more depth of group B and C. The increase of hardness for each group was more rapid within 0.5 h after irradiation, thereafter the hardness increased gradually to maximum at 24 h. At 120 h after irradiation, hardness of group C was greater than that of other two groups at more than 1 mm depth (P<0.001).</p><p><b>CONCLUSION</b>Under dual-cured condition, hardness has significant difference between self-adhesive and universal resin cements, however their hardness development is similar.</p>