ABSTRACT
Objective:To investigate the effects of Porphyromonas gingivalis ( P. gingivalis) fimbrillin (FimA) on the progression of esophageal squamous cell carcinoma (ESCC). Methods:Wild-type P. gingivalis and fimA gene-deleted P. gingivalis ( fimA-/-P. gingivalis) were used to infect ESCC cells after morphology and PCR identification. Immunofluorescence, CCK-8 and Transwell chamber were used to detect the effects of FimA on the infectivity of P. gingivalis and it influences on cell invasion, proliferation and migration. Western blot was used to detect pSmad2/3 changes. The growth of tumor was detected in a nude mouse model bearing subcutaneous tumor. Results:Deletion of FimA might reduce the interbacterial adhesion of P. gingivalis. Compared with wild-type P. gingivalis, less fimA-/-P. gingivalis could infect NE6-T cells. Moreover, the proliferation, migration and invasion of NE6-T and KYSE30 cells as well as the activation of pSmad2/3 induced by P. gingivalis were inhibited after deletion of FimA. The growth of KYSE30 infected by fimA-/-P. gingivalis in nude mice was significantly slower than that of the wild-type P. gingivalis group. Conclusions:FimA mediated the effects of P. gingivalis on promoting the evolution of ESCC and was a potential target molecule to block the tumor-promoting effect of P. gingivalis.
ABSTRACT
Objective To detect the expression levels of vascular endothelial growth factor D ( VEGF-D) , D2-40 and MMP-7 in papillary thyroid carcinoma ( PTC) and to explore their relationship with micro lymphat-ic density, distribution,and cervical lymph node metastasis .Methods SP immunohistochemistry was used to study the expression of VEGF-D, D2-40 and MMP-7 in 38 cases of PTC with cervical lymph node metastasis , 45 cases of PTC without cervical lymph node metastasis , and 22 cases of normal thyroid tissues .Micro lymphatic density ( MLD) was counted .Results The expression level of VEGF-D in PTC with cervical lymph node metasta-sis and normal thyroid tissues had significant difference (χ2 =13.074, P=0.000, P<0.0167).The expression level of D2-40 in PTC with and without cervical lymph node metastasis had significant difference ( P<0.05 ) . MMP-7 expression in PTC with and without cervical lymph node metastasis had statistical significance ( 91.67%vs 73.33%, P<0.05).MLD in PTC with and without cervical lymph node metastasis and in normal thyroid tis-sues had statistical difference (11.7 ±3.5 vs 8.9 ±3.1 vs 3.9 ±2.7, P<0.05).Conclusion VEGF-D, D2-40 and MMP-7 are highly expressed in PTC and they are possibly related with cervical lymph node metastasis of PTC.
ABSTRACT
Purpose:To investigate the expression and clinical significance of estrogen receptor and Cyclin D1 in the differentiated thyrord carcinoma. Methods:We investigated the expression of ER and Cyclin D1 in thyroid normal tissue, thyroid adenoma and DTC by SP immunohistochemical technique. We have collected 89 specimens from surgical resections, and all the sections were formalin-fixed and paraffin-embedded, each has detailed clinical data, two pathologists classified the histopathologic types. DTC 43 cases (papillary thyroid carcinoma, 39 cases, follicular thyroid carcinoma, 4 cases); thyroid follicular adenoma 30 cases, and 16 cases of normal thyroid tissues taken from around the thyroid adenoma. Results:The positive rates of ER and CyclinD1 in the DTC were 53.5%(23/43), 65.1%(28/43) respectively; the positive rates of ER and CyclinD1 in the thyroid adenoma were 26.7%(8/30), 36.7%(11/30) respectively; the positive rates of ER in normal thyroid tissues were 12.5%(2/16), while CyclinD1 were negative. The ER expression in DTC was significantly higher than that in the thyroid adenoma and the normal thyroid tissues(P