ABSTRACT
Objective:To determine the compositions of Pingwei microemulsion through the formula screening and optimization, and carry out the pharmacodynamic validation. Methods:Three levels of mixed volatile oil,dregs water extract and glycyrrhizin were determined by single factor investigation. With the ulcer inhibition rate as an indicator,the orthogonal test was carried out to determine the amount of each component in the formula,and the pharmacodynamic validation was performed on the mouse model of gastric ulcer induced by indomethacin and the rat model of ulcer induced by ethanol hydrochloride.Results:The amount of each component in the formula was determined as follows:SFE-CO2mixed volatile oil of Atractylodes Lancea (Thunb.) DC., Magnolia officinalis Rehd. Et Wils. and Pericarpium Citri Reticulatae was 0.8 ml,glycyrrhizin was 60 mg,and the water extract after SFE-CO2extraction of Atrac-tylodes Lancea (Thunb.) DC.,Magnolia officinalis Rehd. Et Wils. and Pericarpium Citri Reticulatae was 2.0 g. Conclusion: The preparation process of Pingwei microemulsion is feasible,and Pingwei microemulsion has a good anti-ulcer effect,equalling to the posi-tive control Sanjiuweitai.
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OBJECTIVE:To establish a method for bacteria1 endotoxin test for Peramivir hydrate and sodium chloride injec-tion. METHODS:Maximum non-interference concentration of the sample with different batohes were used for interference test. was determined by interference test according to endotoxin gel method stated in Chinese Pharmacopiea (2010 edition)and bacterial en-edotoxintest by tachypleus amebocyte lysate from 2 manufact urers. RESULTS:The interference on bacterial endotoxin test could be excluded when the sample was diluted to 2.5 mg/ml and the limit of bacterial endotoxins was 0.5 EU/ml. CONCLUSIONS:The established method can be used to detect the bacterial endotoxin of Peramivir hydrate and sodium chloride injection.
ABSTRACT
Objective: To observe the clinical effect of sodium nitroprusside and dopamine injected by micro pump for the treatment of refractory heart failure. Methods:One hundred and six cases of patients with refractory heart failure were chosen as the research object, which were cardiac functionⅢ~Ⅳlevel. All patients were treated with sodium nitroprusside and dopamine injected by micro pump. Starting dose of sodium nitroprusside was 6.25 μg/min. At same time pumping dopamine, starting dose of 2 g/kg·min, the dose was increased gradually until blood pressure dropped to 90~100 mm Hg, continuous 7~10 d, the signs and symptoms after treatment and parameters of cardiac function and adverse reactions were observed. Results: 1)In 106 cases of patients with refractory heart failure after treatment, the total effective rate 85.85%(91/106). 2)The heart rate, blood pressure, cardiothoracic ratio, left ventricular short axis reduced rate,cardiac output, each index of cardiac,indexes of left ventricular ejection fraction were improved significantly after treatment. Conclusion:The clinical effect for the treatment of refractory heart failure by using the micro pump injection of small doses of sodium nitroprusside combined dopamine would be obvious, have less adverse reactions, worthy of clinical popularization and application.