The characters of proliferating activity and biosynthetic function of fibroblasts from abnormal scars after complete contact in vitro / 中华整形外科杂志

<p><b>OBJECTIVE</b>This study was to determine the proliferating and biosynthetic characters of fibroblasts from abnormal scars and normal skins after complete contact in vitro.</p><p><b>METHODS</b>6 samples of keloid, hypertrophic scar and normal skin were collected respectively. Proliferation, inhibition and biosyntheses of different fibroblasts were investigated by detecting proliferating cell nuclear antigen(PCNA), P16, type I and III collagen proteins, and Pro alpha 1 (I) and Pro alpha 1 (III) procollagen gene expressions under the condition of fibroblast complete contact by means of cell culture, immunohistochemistry and molecular biological techniques, etc.</p><p><b>RESULTS</b>Overlap and high levels of proliferating activity and biosyntheses of complete contact fibroblasts from keloids indicated that these fibroblasts lost the behavior of contact inhibition and density inhibition. However, proliferating activity and biosynthetic function of complete contact fibroblasts from normal skins reduced dramatically. Biosynthetic function of complete contact fibroblasts from hypertrophic scars was still very strong, but proliferating activity was lower than keloid and higher than normal skin.</p><p><b>CONCLUSION</b>Proliferating and biosynthetic characters of complete contact fibroblasts from abnormal scars and normal skins were different, which may play an important role in the mechanisms of abnormal scar formation.</p>

Effect of IL-?1 on EGF, EGFR and TGF?R_1 in fibroblasts of scars / 中华医学美学美容杂志

0.05). After IL-1? was addied, densities of EGF, EGFR, TGF?R 1 were 224.00?31.59, 178.67?27.86 and 80.34?11.75 respectively in fibroblasts of normal skin; 128.75?18.31, 105.82?21.61 and 109.83?25.79 in fibroblasts of HS;113.01?24.71,93.34?30.71 and 118.75?19.27 in fibroblasts of keloid. Densities of EGF and EGFR in fibroblasts of normal skin was significantly higher than those in HS and keloid (P

Nestin-positive cells isolated from human fetal pancreases / 中国微创外科杂志

Objective To investigate whether nestin-positive cells can be isolated from human fetal pancreases and cultured in vitro for passages. Methods The islet-like cell clusters (ICCs) were isolated from pancreases of induced human fetuses at 16, 18, 20 gestational weeks and cultured subsequently. Immunohistochemical staining and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the expression of the nestin, pancreatic and duodenal homeobox gene-1 (PDX-1/IPF-1), islet endocrine hormone insulin and glucagon. Results Nestin-positive cells, which may further transform into sphere cell clusters that include PDX-1, insulin and glucagon expressing cells, as well as nestin-positive cells, were isolated from the ICCs in vitro and cultured with several passages. Conclusions The nestin-positive cells can be isolated from fetal pancreases, and may have the capability to proliferate and differentiate into islet endocrine cells.