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Objective To explore the clinical significance of changes of serum IL-6,IL-8 and adiponectin in patients with acute cerebral infarction(ACI).Methods Serum IL-6,IL-8 and adiponectin levels were measured by ELISA in 98 patients with ACI and 80 healthy control.Results The ACI group serum IU6 and IL-8 levels were higher than the healthy control group(all P＜0.01),while the level of adiponectin was converse;The levels of serum IL13,IL-18 increased gradually with the increase of the severity and the volume of ACI,while the level of adiponectin was decreased;the serum level of adiponectin was negative correlated with the level of IL-6、IL-8 in patients with ACI (r=0.733,0.715,all P＜0.05),the serum level of IL-6 was positive correlated with the level of IL-8(r=0.830,P＜0.01).Conclusion The serum IL-6,IL-8 and adiponectin could reflected situation of patients and extent of neurologic impairment,which had close relationship with the clinical process of ACI and had significance value to evaluate the pathogenetic condition and prognosis.
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Objective To optimize the extracting conditions of polysaccharide from Imperata Cylindrica and establish a suitable method for its content determination. Methods Based on the qualitative yield of polysaccharide extraction, a orthogonal test was employed to evaluate the effect of four factors including extracting temperature, extracting time, the extraction times and the ratio of material to liquid. The phenol-dense sulphuric acid method was applied for the content determination of polysaccharide. Results The times of extraction and the extracting temperature influenced the content of polysaccharides mostly, while the duration of extraction and the amount of water showed little influence. Conclusions The optimum extraction condition was refluxing and extracting for 3 times at 85 ℃ for 3 hours, and with 15 folds of water for every time. The contents of polysaccharide in Imperata Cylindrica collected from two different places were above 0.15%.
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stir-baked Radix Paeoniae Alba with vinegar. Conclusion Different processing method has great influence on the paeoniflorin content of Radix Paeoniae Alba.
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Objective To investigate the quality of Pulsatilla chinensis from different sources on reference of pulchinenoside B4 as the index. Methods Five kinds of Pulsatilla chinensis herbs and cut crude drug were purchased from Chinese herbal medicine market in Bozhou,and Wild Pulsatilla chinensis were collected from Chuzhou. The content of pulchinenoside B4 were determined by HPLC. Results Linear relationship was good within 0.312 5～10 ?g,the regression equation was Y=276 350X -12 180,R2= 0.999 6. Conclusion The differences of pulchinenoside B4 from different sources of Pulsatilla chinensis varied greatly.
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Objective: To optimize the alcohol-extraction process and condition of Radix Pulsatillae.Methods: Alcohol concentration,the extraction time and number of extraction on effect of extraction rate of pulchinenoside B4 were evaluated by orthogonal design L9(34).Results: The optimum condition of the alcohol extraction was: 75% ethanol,3 times of extraction,3h for extraction process.Conclusion: The extraction method can be used for reference for the extraction process of Radix Pulsatillae.