ABSTRACT
Objective] To explore the clinical experience of Ma Weiming in the treatment of insomnia.[Method] By following the teacher clinic, the author summarizes chief physician Ma Weiming’s academic experience of treatment of insomnia with classical prescriptions. [Results] In Ma Weiming's opinion, everything harasses the heart, mind vacillation, inducing liver ’s function of storing soul abnormal, the spirit restless is due to insomnia with much dream. So teacher Ma pays attention to “dream diagnosis”.The treatment of insomnia must be combined with heart and liver, making the mind quiet and the liver comfortable. He likes using flowers to balance Qi. In Ma Weiming's opinion, collaterals are invaded by prolonged illness, due to blood stasis. The treatment should use insect drugs to dispell wind and remove obstruction in the meridians, purifying orifices, promoting sleep. [Conclusion] According to the etiology of insomnia to distinguish among different dreams, teacher Ma uses classical prescriptions and combines with his own unique clinical experience on treating insomnia, which has significant effect and worth generalizing.
ABSTRACT
The alpha-hemolysin protein of Staphylococcus aureus, which was expressed in Escherichia coli BL21 (DE3) with recombinant pET32a(+)-alpha-HL plasmid, was purified with gel filtration chromatography (GFC) and Ni-NTA spin columns. The quality and biological characteristic were compared. First, the purified products were analyzed with SDS-PAGE, and the expected protein band was with a molecular mass of 53 kD. Second, protein concentration was determined by the method of Bradford, and the median hemolytic dose potency (HD50) was finally analyzed with rabbit erythrocyte. The protein purified with GFC was 0.337 mg/mL, its hemolysis activity was 1519 HU/mg, and hemolysin yield was 14.04%. Meanwhile, the protein purified with the Ni-NTA Spin Columns was 0.35 mg/mL, its hemolysis activity was 1463 HU/mg, and hemolysin yield was 17.5%, respectively. The results showed that there is no significant difference in the quality, hemolysis activity and yield of the recombinant proteins purified with Ni-NTA spin columns and GFC.
Subject(s)
Chromatography, Gel , Methods , Escherichia coli , Genetics , Metabolism , Hemolysin Proteins , Genetics , Nitrilotriacetic Acid , Organometallic Compounds , Recombinant Proteins , Genetics , Staphylococcus aureus , Genetics , MetabolismABSTRACT
Objective:? -Hemolysin of Staphylococcus aureus,which was expressed in E.coli BL21(DE3) with recombinant pET32a+-?-HL plasmid,was purified with gel filtration chromatography(GFC),and then the engineered subunit vaccine was developed. The immunity effectiveness of this vaccine was evaluated on mouse models.Method:The purified fusion protein was analyzed in SDS-PAGE,and subjected to the evaluation of its median hemolytic dose potency(HD50) was finally analyzed with rabbit erythrocyte. Protein concentration was determined by the method of Bradford. Antibody titers were evaluated on ELISA,and then challenged to gain the immunity protect index.Results:There is an expected protein band with molecular mass of 53kDa in SDS-PAGE,and the concentration is 0.1278mg/ml. The hemolysis activity is 8012.5 HU/mg. There are specific antibodies acquired in blood-serum from mice after vaccined and the antibody titers rising until it has arrive the max,then following down.Conclusion:The purified fusion protein has good fineness and hemolysis activity,the antibody titers initiated by the protein vaccine go with regulation and the immunoprotection is satisfied.
ABSTRACT
A preliminary experimental research on the proliferation and differentiation of rat bone marrow mesenchymal stem cells with exposure to 50 Hz magnetic fields is done. The experimental results can be well repeated, showing that 50 Hz magnetic fields can evidently affect the proliferation and differentiation of rat bone marrow mesenchymal stem cells (MSCs), besides, the results are not linear with the amplitude of magnetic fields, but existing therein a 'window' phenomenon. At the end of this paper, the domestic and overseas development of the research is discussed on with the emphasis how to cure the bone fracture with the help of magnetic fields, and our investigative thoughts are also presented.