Correlation study between CD64 +neutrophils and infection of patients with burns / 医学研究生学报

Objective The in vitro studies of indicators in burn patients with complicated infection have been little studied till now.So this study aims to investigate the change of proliferation of CD64 +neutrophils from the peripheral blood of burn patients in vitro. Methods CD64+neutrophils from peripheral blood of healthy people were isolated and purified, which was followed by stim-ulating its proliferation with inactivated Staphylococcus in vitro.We further analyzed the proliferation index with Modfit 2 analysis soft-ware.86 burn patients were divided into two groups, 44 cases with complicated infection assigned to experimental group, 42 uninfected assigned to controls.We further detect the counts of WBC and the percentage of CD64 +neutrophils, and then analyzed the specificity and sensitivity by using the receiver operating characteristic ( ROC) curves. Results This in vitro study, the average proliferation index of CD64 +cells in experimental wells was significantly higher than controls (6.48 ±0.11 vs 2.63 ±0.02), the difference was statistically significant (P<0.05);the percentage of CD64 +cells in the peripheral blood of patients in experimental group(64.25 ± 13.11%) was significantly higher than patients without infection(16.33 ±2.77%);The sensitivity and specificity of diagnostic meth-od of CD64 +cells for the burn infection were respectively 94.2%and 76.8%, which was superior to the traditional diagnostic meth-od of WBC ( 68.5%, 64.7%) according to ROC curves. Conclusion CD64 + cells in peripheral blood of burn patients complicated by infection increased more significantly and earlier when compared with the traditional diagnostic method, which may be used as a useful diagnostic indicator for burns complicated infection.

Protein and C_(3b) Fc receptor expression of the macrophage in autotranspla ntated splenictissue / 中国普通外科杂志

Objective To investigate the f un ction of the autotransplanted splenic tissue. Method s 120 Kunming mice were randomly divided into two groups: sham operation group and group of 50% autograft splenic tissue implantation in the om ental pouch after total splenectomy. Six months after, splenic transplants were removed, and C 3b and Fc receptor and the expression of protein on the macr ophages was assayed in the implanted splenic tissue. Results The expression of protein and the receptor on the mac rophage in transplants were similar with that in the normal group. Conclusion The function of the macrophages in t he implanted splenic tissue judged by the expression of protein and receptor is normal, the autotransplanted splenic tissue can fulfil the function of the norma l spleen.

Experimental study: Umbilical cord vein used as an arterial conduit with a long term follow follow up / 第三军医大学学报

This paper reports the umbilical cord vein (UCV) used as the aotic grafts for dogs and the result o a long term follow-up . The UCVs are treated with 10% formalin. All grafts with follow-up 2-3. 5 years are patent. The lumen at the anastomotic site is slightly narrower and that of the grail is braoder. By the light and electron microscopy the inner wall covered with endothelium is observed . The endothelial cells proliferate actively and range regulary . There are more collagen and a few fibrocytes and myofibroblasts myointimal cell in the intima, but quite few smooth muscle cells in the medium . In conclusion , the umbilical cord veins are convenient for the moderate arterial grafts .

Morphology and structure of the vessels of the abdominal skin flap in rats / 第三军医大学学报

There are 2 sets of arteries supplying the abdominal skin flap of the rat.The medial set consists of the medial branch of the lateral thoracic artery,the superficial epigastric artery and the perforating arteries and the lateral set is composed of the lateral branch of the lateral thoracic artery,the superficial circumflex iliac artery and the branches of the lumbar arteries.The former supplies the medial 2 thirds and the latter the lateral third of the abdominal wall.There is no significant difference in the number of vessels and the area fraction between the medial and lateral anastomotic areas.The abdominal skin flap of the rat is thinner than the human one and contain a little amount of fat and a layer of panniculus carnosus.The vascula-ture of the abdominal skin flap can be divided into 5 layers,that is,the superficial fascial,the pannicular carnosal,the profound dermal,the subpapillary and the papillary layers,which is similar to the vasculature of the human abdominal flap.But in the rat,there is no arterial anastomosis in the reticular layer of the dermis of the abdominal flap,which might be relatded to the insufficient thickness of the flap of the rat.

THE ANGIOARCHITECTURE OF SKIN FLAP ON THE ABDOMEN / 解剖学报

A study on abdominal skin flaps of the fresh cadavers of young men under the operation, light and scanning electron microscope, and MAS image analysis system has been made. Methods include Chinese ink, ABS or methyl methacrylate injection and histological slides, transparent specimens as well as casts preparation. The course of the vessels in the human abdominal skin flap is in the "three steps form". Trunks and main branches of the cutaneous vessels run in the deep part of the superficial fascia, their branches and terminals form anastomoses in the middle of the reticular layer of dermis, and branches from the anastomoses form microvascular network in the subpapillary layer. The vascular networks in the skin flap are concentrated in five layers: the deep fascial, superficial fascial, profund dermal, subpapillary, and papillary layers. Between the subpapillary and the profund dermal vascular networks, there is a layer devoid of vascular network and crossed by vascular arteries only. Therefore, this layer may be called as "vascular network devoid area". The differences of the area fraction (Aa) or number of vessels are not significant among various areas of the abdominal skin flap. Design in detaching the abdominal skin flaps and skin grafts is discussed.

APPLIED ANATOMY OF THE BLOOD VESSELS OF SCIATIC NERVE / 解剖学报

The blood supply and source about the sciatic nerve in 20 fetuses were studied. We observed the number and diameter of the branches of blood vessels and the size, characteristic distribution of the network formed from them, microscopically and by naked eye. A combined angiography was used. After injection of Chinese ink the epineuria were peeled. About the vascular distribution of the nerve tract we perceived that:1. The blood supply of the sciatic nerve was from two vascular systems——external and internal (separated by the perineurium); 2. The external part showed segmental of multiple origination, mainly from the nutrition arteries, and some arterioles directly from the sciatic arteries. The number, diameter, branching type of the vessels, size and shape of the anastomosing network were also observed; 3. The internal part consisted of microarteries and capillaries, which form an anastomosing network. The diameter of the vessels, shape and size of the network were measured.In view of the above-mentioned data, when a part of the sciatic nerve should be isolated, the clinical physicians ought to consider the blood supply in segmental character, so that the part of nerve taken off must not be too long to avoid injuring the nutritional vessels.

OBSERVATIONS ON THE APPLIED ANATOMY OF THE SPLENIC ARTERY / 解剖学报

The splenic arteries of 110 Chinese cadavers and 65 dogs were observed with methods nf gross dissection, angiography (suspension of red lead oxide (Pb_3O_4) as the contrasr meduim) and corrosive cast (Polychloroethylene). The ramifications of the splenic artery and their relation to the splenic lobes and segments were studied. The results were outlined as follows:1. There are three patterns of splenic artery ramifications in human: Type Ⅰ, biramification(89%); Type Ⅱ, triramification(8%); and Type Ⅲ, polyramification(3%).2. In type Ⅰ, most of the splenic arteries divide into a superior and an inferior splenic lodar arteries. Most of the superior splenic lobar arteries subdivide into the superior and mid-superior segmental arteries and the inferior splenic lobar artery subdivides into the mid-inferior and inferior segmental arteries.3. All of the splenic arteries of the dogs we studied may divide into two splenic lobar arteries and each lobar artery further divides into two segmental arteries without exception.4. Between the lobar or segmental arteries there are zones poorly vascularized.5. Based on the anatomic observations we had performed experimental partial splenectomies on 15 dogs. All of the dogs survived the operation and their wound made on the spleen healed up very well.