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Objective To investigate the clinical characteristics,diagnosis and treatment of pelvic abscess complicated with septic shock.Methods The clinical symptoms,laboratory tests,surgical procedures,and prognosis were analyzed retrospectively for 9 patients with pelvic abscess complicated with septic shock.All the patients were treated in the Second Affiliated Hospital of Soochow University from August 2009 to March 2017.Results High fever and abdominal pain were reported in all the 9 patients,associated with hypotension,cardiac,pulmonary and renal dysfunction,and other symptoms.Eight patients were referred to ICU after early volume resuscitation,antimicrobial treatment,surgery or puncture.These 8 patients finally survived.The remaining one patient experienced multiple organ failure,disseminated intravascular coagulation,and extensive bleeding during treatment,and died finally.Conclusions Early diagnosis,adequate volume resuscitation,antimicrobial therapy,appropriate vasoactive drugs,and timely surgical removal of abscess are important for improving the survival of patients with pelvic abscess and septic shock.
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Objective To investigate the expression and relationship of Jab1 and P27kip1 in ovarian cancer cell HO-8910. Methods Cells were treated with serum starvation and release.The expression and distribution of Jab1 and P27kip1 were detected by western blot and subcellular fractionation.HO-8910 cells were transfected in vitro with pcDNA3.1-MYC-Jab1.Western blot as well as subcellular fractionation was used to detect the expression and localization of P27kip1.Results The growth of HO-8910 cells was blocked by serum starvation.P27kip1 increased while Jab1 decreased.The reverse changes were found after serum release.P27kip1 and Jab1 could form compound in HO-8910 cells detected by immunoprecipitation.48h after transfected by pcDNA3.1-MYC-Jab1,the expression of P27kip1 decreased and the distribution of P27kip1 translocated from nucleus into cytoplasma in HO-8910.Conclusion Jab1 may control the location and expression of P27kip1 through integrating with P27kip1,and participate in regulating the growth of ovarian cancer cells through interfering with the function of P27kip1.
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Objective To explore the clinical value of myomectomy and hysterectomy under laparoscope.Methods Fifty-eight cases of gynecological laparoscopic surgery were analyzed retrospectively,including 30 cases of myomectomy and 28 cases of classical intrafascial supracervical hysterectomy(CISH) or laparoscopic assisted vaginal hysterectomy(LAVH). Results The laparoscopic operation was successfully completed in all the 58 cases.The operation time was 89.4?12.1 min for myomectomy and 108.5?10.2 min for hysterectomy.On the second postoperative day,the decrease rate of hemoglobin was 11.3%?3.3% and 14.2%?3.2%.The length of hospital stay was 5.6?0.2 d and 6.6?0.5 d.Postoperative follow-up in the 58 cases for 3～17 months(mean,11.4 months) found 2 cases of complications(3.4%). Conclusions Laparoscopic myomectomy and hysterectomy offers little invasion and rapid recovery.