5′/3′ Imbalance Strategy for qRT-PCR to Detect ALK Fusion Mutation in Primary Lung Adenocarcinoma in Gannan Region / 肿瘤防治研究

Objective To explore the characteristics of ALK fusion gene in patients with primary lung adenocarcinoma in Gannan region, with hopes of scientifically guiding such patients towards selecting targeted drugs. Methods 5′/3′ imbalance strategy for qRT-PCR was used to detect the expression of ALK fusion gene in 233 cases of primary pulmonary adenocarcinoma and the clinical pathological characteristics were analyzed. Results The expression rate of ALK fusion genes was 9.01% (21/233). The expression rate of ALK fusion gene in female and N1-3 patients was significantly higher than that in male and N0 patients (P < 0.05); The expression rate of ALK fusion gene in patients with no smoking history, age < 55 years, M1 stage and fresh biopsy specimens was relatively high (P > 0.05); The expression of ALK fusion gene was not correlated with TNM stage, T stage, surgical treatment or tumor distribution location (P > 0.05). Moreover, in the subgroup analysis of 153 M1-stage cases, no correlation was found between the expression of ALK fusion gene and the metastasis of common sites of lung cancer (bone, brain, opposite lung, pleural dissemination adrenal glands and liver) (P > 0.05). Conclusion The expression rate of ALK fusion gene in primary lung adenocarcinoma in Gannan region is relatively high, which is common in female patients with lymph node metastasis.

Analysis of clinicopathological characters for IgG4-related disease / 临床与实验病理学杂志

Purpose To study the clinicopathological features and immunohistochemical characteristics of IgG4-related disease. Meth-ods The microscopic characteristics and immunohistochemical staining (EnVision) of IgG, IgG4, CD138 and CD34 have been per-formed on 12 cases of IgG4-related disease. Results IgG4-related disease were characterized by diffuse fibrosis, accompanied with in-filtrating of dense lymphocytes and plasma cells surrounding neurovascular and occlusive phlebitis. Immunohistochemical staining re-sults showed the ratio of IgG4+/IgG+ cells were over 40%. Conclusions IgG4-related disease is absent of characteristic clinical and radiographic features and is easily misdiagnosed as tumor. Preoperative serum IgG4 detection could be used as the prior examina-tion for the suspected cases.