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Objective:To evaluate the efficacy for nanoscale microneedle injection of compound betamethasone combined with 308 nm excimer laser in the treatment of stable vitiligo patients.Methods:A total of 80 patients with stable vitiligo were enrolled in Guangzhou Dermatology Hospital from May 2018 to May 2020. There were 40 patients (21 males and 19 females) in control group, aged 17-65 (32.4±1.7) years, and 40 patients (20 males and 20 females) in observation group, aged 18-67 (28.7±1.8) years. The control group was treated with compound betamethasone injection packet combined with 308 nm excimer laser. The observation group was treated with nanoneedle injection of compound betamethasone combined with 308 nm excimer laser. We compared the clinical efficacy and incidence of adverse reactions between the two groups.Results:Comparison of clinical efficacy showed that after 3 months of treatment, the total effective rates of the observation group and the control group were 80.00% and 67.50%, respectively, with significant difference (χ 2=4.560, P<0.05). After 3 months of treatment, the white spot area of the control group was (9.89±1.65) cm 2, which was significantly higher than that of the observation group (7.83±1.78) cm 2 ( t=5.370, P<0.05). Conclusions:The nanoneedle injection of compound betamethasone combined with 308 nm excimer laser in the treatment of stable vitiligo is effective and safe.
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Objective@#To analyze the epidemiological characteristics and influencing factors of norovirus aggregation in primary and secondary schools in Haidian District of Beijing from 2015 to 2018,and to provide scientific basis for the prevention and control of school aggregation epidemic situation.@*Methods@#An epidemiological method was used to analyze the epidemic characteristics of norovirus aggregation in the primary and secondary schools in Haidian District of Beijing from January 2015 to December 2018. Multivariate Logistic was used to analyze the influencing factors of norovirus aggregation.@*Results@#A total of 74 cases of norovirus aggregation in primary and secondary schools in Haidian District of Beijing from 2015 to 2018 were reported, involving 52 primary and secondary schools. Totally 1 291 suspected cases and a median of 12.5 cases of single epidemic were reported. Winter and spring were high incidence season. There was significant difference in the incidence rate of epidemic situation in primary and secondary schools(χ2=98.82, P<0.01). There was significant difference in the incidence rate between men and women(χ2=55.56, P<0.01). A total of 648 samples and 486 positive samples were collected, with a positive rate of 75.00%, mainly GⅡ virus, accounting for 87.45%. Multivariate Logistic regression showed that the scale of school(>2 000，1 000~2 000), student’s hand hygiene habit,requirement for medical care after the outbreak is the influence factor of the outbreak(OR=3.78，2.95，8.25，2.88).@*Conclusion@#Poor hand hygiene habits are the most important risk factors for the outbreak, so schools and parents should cultivate children to develop good hygiene habits of washing hands before and after meals, and timely isolation of cases found in schools is also an important measure to prevent and control the epidemic.
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Objective To determine the expression of miRNA-148a-3p in CD4+ T lymphocytes in peripheral blood of patients with psoriasis vulgaris,and to explore its role in occurrence of psoriasis vulgaris.Methods Totally,20 patients with psoriasis vulgaris and 20 healthy controls were enrolled from Guangzhou Institute of Dermatology between July 2017 and April 2018.Peripheral venous blood samples were obtained from these subjects,and CD4+ T lymphocytes were isolated from these peripheral blood samples by magnetic cell sorting system.Real-time quantitative PCR (RT-PCR) was performed to determine the expression of miRNA-148a-3p in CD4+ T lymphocytes in the peripheral blood.Potential target genes of miRNA-148a were predicted by using bioinformatics software,and verified by using a dual-luciferase reporter system.Western blot analysis was conducted to determine the protein expression of Bcl-2 interacting mediator of cell death (Bim,the potential target gene of miRNA-148a-3p) in the CD4+ T lymphocytes of the subjects.Statistical analysis was carried out with SPSS 20 software by two sample-t test for comparing the means of normally distributed data,and by Pearson correlation analysis for analyzing the correlation of two variables.If the data were not normally distributed,Mann Whitney U test was used for comparing means between two groups,and Spearman correlation analysis for analyzing the correlation of two variables.Results The miRNA-148a-3p expression in the CD4+ T lymphocytesin the psoriasis vulgaris group (18 cases,5.61 ± 1.66) was significantly higher than that in the healthy control group (12 cases,1.00 ± 0.26;U =12,P ＜ 0.05),and was positively correlated with the psoriasis area severity index (PASI) score (r =0.93,P ＜ 0.001).Bim was predicted to be one of the potential target genes of miRNA-148a-3p by bioinformatics software,which was also verified by using a dual-luciferase reporter system.The protein expression of Bim in the CD4 + T lymphocytes was significantly lower in the psoriasis vulgaris group (11 cases,0.69 ± 0.07) than in the healthy control group (8 cases,0.93 ± 0.06;t =4.38,P ＜ 0.01),and the protein expression of Bim in the patients with psoriasis vulgaris was negatively correlated with PASI score (r =-0.774,P ＜ 0.01).Conclusion miRNA-148a-3p is overexpressed in CD4+ T cells in the peripheral blood of patients with psoriasis vulgaris,which may regulate the protein expression of Bim,leading to abnormal activation of CD4+ T cells,and then participate in the occurrence and development of psoriasis.
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When the pathogen infects the fetus,the pathogenic microorganism and the infection product are recognized by the corresponding receptor.The fetus innate immune system is passively activated,which produces proinflammatory cytokines,induces cascade reaction of cytokines,and releases a large number of inflammatory factors secreted by the body.Its toxic effect can cause damage to the brain,lung,small intestine and heart and other important organs of the whole body,which seriously threatens the life of the perinatal infants and their subsequent survival quality.It has been found that fetal cardiovascular system is one of the important target organs of intrauterine infection.Cytokines produced by cardiac inflammation and induced by intrauterine infection can damage myocardial cells,affect the proliferation of myocardial cells,and cause damage to cardiac function.Moreover,the persistent influence of infection on fetus leads to fetal vascular remodeling and changes in fetal cardiopulmonary hemodynamics.This article reviews the effects of pathogens of intrauterine infection and fetal cardiac inflammation,cardiac hemodynamics,cardiomyocyte development,gene program of cardiomyocyte and cardiac structure development on fetal cardiovascular system.
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Objective To evaluate the efficacy and safety of microneedling radiofrequency in the treatment of facial acne scars.Methods A retrospective study was conducted.According to the inclusion and exclusion criteria,42 patients with acne scars receiving microneedling radiofrequency treatment (observation group) and 47 patients with acne scars treated with fractional CO2 laser (control group) in Guangzhou Institute of Dermatology were enrolled into this study from June 2016 to June 2017.Single-blind evaluation was conducted according to a four-grade scoring system and ECCA grading scale (échelle d'évaluation clinique des cicatrices d'acné) by two clinically experienced dermatologists to evaluate and compare the clinical efficacy between the 2 groups,based on the clinical photos before and after the treatment.Statistical analysis was done by using chi-square test,t test of two independent samples and paired Wilcoxon signed rank test.Results After treatment,no significant difference in the total response rate was observed between the observation group (92.9％,39 cases) and control group (89.4％,42 cases;x2 =0.042,P ＞ 0.05).Before the treatment,there was no significant difference in the ECCA score between the observation group (46.7 ± 16.3) and control group (45.7 ± 15.8,t =0.271,P ＞ 0.05).After the treatment,the ECCA score in the observation group and control group significantly decreased into 29.5 ± 15.1 and 31.3 ± 14.9 respectively (Z =-5.713,-6.086,respectively,both P ＜ 0.05).Conclusion Microneedling radiofrequency is effective for the treatment of facial acne scars,with high safety and less injuries.