Problems and Countermeasures of Innovative Development of Southern Medicine Industry in Zhaoqing City / 中国实验方剂学杂志

Zhaoqing city is located in the midwest of Guangdong province,the middle and lower reaches of the mainstream of the Xijiang river.Many species of southern medicines grow very well in the Xijiang river basin of Zhaoqing because of existing of enormous mountainous and hilly areas,loose and fertile soil,warm climate,as well as abundant sunshine and rain.Zhaoqing has a long history of cultivating some species of southern medicines.For example,Cinnamomi Cortex was planted more than 300 years ago in Zhaoqing.Under the advocacy of national policy,the planting scale of some species of southern medicines expanded rapidly since 1980s.Up to now,plantation of the 4 species of southern medicines,namely,Morindae Officinalis Radix,Citri Sarcodactylis Fructus,Polygoni Multiflori Radix and Cinnamomi Cortex,has developed into a important representative industry in Zhaoqing.Zhaoqing also develops into the genuine producing area of this 4 species of southern medicines currently,and these products are sold well at home and abroad.Although great progress has been made in southern medicine industry in Zhaoqing,there still exist lots of problems for its future development,such as scattering planting instead of overall program,impure germplasm resources,plant diseases and insect pests,pesticide residue and pollution of heavy metal,lack of quality control in trading process,as well as absence of deep-processing and additional value of products.This paper analyzes the development status,advantages and problems of southern medicine industry in Zhaoqing,and puts forward some corresponding measures and suggestions.These proposals may have important guiding significance for promoting the development of local southern medicine industry.

An Investigation of the Situation of Chronic Diseases and the Effects on Quality of Life Among Different-ages Elderly in Community / 昆明医科大学学报

Objective To investigate the situation of chronic diseases and the effects on quality of life among different-aged elderly in community and provide evidence for targeted improvement quality of life.Methods During March to July 2015, the elderly who was aged 60 and over as respondents from two communities in Kunming were enrolled. Cross-sectional study and cluster sampling were used based on Activities of Daily Living scale (ADL) and Balthel index.questionnaire was designed and information of chronic disease in the elderly was colected.The effects on Balthel index in different chronic diseases and age groups were analysed.Results A total of 589 subjects were investigated, chronic disease prevalence was93.70％, and the highest prevalence is hypertension that was 27.50％.The quality of life was worse in the elderly who suffered from three kinds of chronic diseases and more older. The quality of life in different age groups elderly was statistically significant (P ＜ 0.05).Conclusion The quality of life in the community elderly is relate with age and the type of chronic diseases.In order to improve their quality of life health guidance should be strengthened.

Expression of Insulin-Like Growth Factor Binding Protein 7 Gene in Multiple Myeloma Cell Line and Methylation Regulation / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To investigate the expression of insulin-like growth factor binding protein 7 (IGFBP7 ) gene in multiple myeloma cell line U266, and study the effect of 5-aza-2'-deoxycytidine (5-aza-dc) on proliferation of U266 cells.</p><p><b>METHODS</b>multiple myeloma cell line U266 was cultured in vitro. The bone marrow mononuclear cells from healthy persons(N-BMMNC) were collected and used as normal controls. The IGFBP7 mRNA expression of U266 cells and N-BMMNC were detected by real-time fluorescence quantitative PCR, the DNA methylation status of the IGFBP7 CpG island was measured by using methylation-specific PCR(MSP). The different concentrations of 5-aza-dc (5 µmol/L, 10 µmol/L, 20 µmol/L) were used to treat U266 cells for 48 hours, the RT-PCR and Western blot were used to detect the effect of IGFBP7 mRNA and protein expressions, the cell growth curve and Annexin V/PI were analyzed by flow cytometry.</p><p><b>RESULTS</b>As compared with normal BMMNC, the lower expression of IGFBP7 gene was found in U266 cells, the obvious hypermethylation of the CpG island in the IGFBP7 promoter was observed. After treatment of U266 treating with different concentrations of 5-aza-dc, the IGFBP7 mRNA expression was up-regulated dose-dependently(P<0.05), the U266 cells grew slowly and apoptosis rates were enhanced dose-dependently.</p><p><b>CONCLUSION</b>As the hypermethylalion of CpG island in IGFBP7 promoter is a frequent event in lower expression of IGFBP7 gene in U226 cells, the 5-aza-dc can up-regulate the expression of IGFBP7 , and can inhibit cell proliferation through induction of cell apoptosis and arrest of cell cycle.</p>

Genome sequencing and analysis of a peste des petits ruminants virus isolate, China/Tib/07 / 病毒学报

Peste des petits ruminants virus is a member of Morbillivirus Paramyxoviridae. The complete genome of a Peste des petits ruminants virus (PPRV) isolate, China/Tib/07 was sequenced and molecular characteristics was analyzed. The internal sequences of the virus genome were amplified by RT-PCR with primers designed according to the published data in GenBank, while the sequences of the 3' and 5' ends of the genome were determined by RACE. Amplification products were directly sequenced,assembled and analyzed with DNAStar4.0. Results showed that China/Tib/07 genome consisted of 15 948 nucleotides in length, encoding six structural proteins and two non-structural proteins just like other known PPRV genomes. Phylogenetically, the virus genome shared homology of 91.6%-98.1% with Southwest Asian isolates among PPRV strains and the highest homology of 64.3% with rinderpest virus among morbillivirus members.

Detection of pim-1 mRNA in prostate cancer diagnosis / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Pim-1 plays an important role in the apoptosis, proliferation, differentiation of cancer cells and progression of cancer. In this study we detected the expression of pim-1 mRNA in normal prostate, benign prostatic hyperplasia (BPH), and prostate cancer (PCa) and explored its diagnostic value for PCa.</p><p><b>METHODS</b>The prostate tissues were collected from 23 patients with PCa, 37 patients with BPH, and 3 healthy volunteers. Pim-1 mRNA expression levels in these samples were determined by the quantitative real-time PCR (QRT-PCR). The differences of expression were calculated based on a standard curve.</p><p><b>RESULTS</b>The ratio of pim-1 mRNA to beta-actin in the normal prostate, BPH, and PCa were 1.05 +/- 0.04, 2.57 +/- 0.74 and 4.45 +/-0.63, respectively. The differences among PCa, BPH and NT were significant (P < 0.05, respectively).</p><p><b>CONCLUSION</b>Detecting pim-1 mRNA expression by QRT-PCR provides a reliable metric for the diagnosis of PCa.</p>

The construction of cDNA expression library from the tentacles of Sagartia rosea / 生物工程学报

A cDNA expression library of the tentacles of Sagartia rosea was constructed. The cDNA was cloned into eukaryotical expression plasmid pcDNA3. SMART protocol was used for cDNA library construction and bioinformatics analysis was carried out. 71 novel EST clones were obtained from 130 sequences in the library, of which there were 21 full-length clones, including cytolysin genes, flourescent protein, ubiquinol-cytochrome C reductase gene, elongation factor, ferritin gene riboflavin kinase gene, ribosomal protein. This provides a base for further investigating their biological activity and application.

Investigation on Dose Levels of Medical Exposure From X-Ray Diagnostic Procedures in Qinghai Province / 环境与健康杂志

Objective To investigate the exposure dose levels of patient body surface and main sensitive organs from routine medical X-ray diagnosis so as to offer a basic data of guidance levels in radiological diagnosis practice. Methods To measure the skin surface exposure doses of the irradiation field and the main sensitive organs of patients by thermoluminescent dosimeter moreover to estimate the main sensitive organs exposure doses using tissue percentage depths formula. Results The exposure dose of chest fluoroscopy was 30 times that of fluorography. The exposure dose(12.41 mGy/time) of barium meal examination in alimentary canal was the highest in all examination. The dose of thyroid(0.210 mGy/time) was the highest when getting the chest fluoroscopy the dose of ovary was the highest when getting barium meal(0.390 mGy/time) and lumbar fluoroscopy(0.168 mGy/time) in all of sensitive organs dose. Conclusion Chest fluorography will give the patients a lower dose exposure of X-ray compared with fluoroscopy.