ABSTRACT
Aim To investigate the therapeutie effect thritis (CIA) rats based on the variation of gut micro- of active ingredient group ( AIG) in the compounds biota.Methods CIA model rats were induced by in- from Periploca forrestii Schltr.on collagin-induced ar- jection of emulsion containing bovine type II collagen.anrl enzyme-linked immunosorbent assay ( ELISA) was used to investigate the changes of TNF-a and IL-6 in ankle joint of rats in each group.Meanwhile, the gut microbial communities were measured using 16s rRNA high-throughput sequencing technology and its function was predicted by KEGG.Results AIG could signifi¬cantly restrain inflammatory cell infiltration and tissue proliferation in synovial tissues of CIA rats,and allevi¬ate joint swelling while significantly reducing the con¬tents of IL-6 and TNF-a in ankle joint of CIA rats.The analysis of gut microbial communities showed that AIG could increase the relative abundances of Bacte- roidetes, Alloprevotella and Prevotella _9 , and decrease the relative abundances of 1 1 genera such as Dubosiel- la, Lachnospiraceae_NKAA 136_group, etc.in CIA rats.The functional prediction showed that AIG coulrl influ¬ence apoptosis and other signaling pathways.Conclu¬sions AIG can obviously alleviate joint swelling, re¬strain inflammatory cell infiltration and tissue prolifera¬tion in joint synovium of CIA rats,and repair the disor¬dered gut microbiota structure of CIA rats, while affecting its apoptosis pathway and restore the body immunity.
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the clinical value of 3D visualization method for simulating percutaneous transcatheter closure of atrial septal defect (ASD).</p><p><b>METHODS</b>3D volume render method was used for visualizing ASD and surrounding structures and 3D modeling method was applied for simulate the shape of occlusion device. The size and the distance between the lower edge of device and atria-ventricular valve of simulation occluder and actual selected atrial septal occluder (ASO) were compared in 30 patients underwent successful transcatheter closure of ASD.</p><p><b>RESULTS</b>The location, geometry, size, extent of ASDs in children were displayed in 3D visualization. No significant difference was found between simulation occluder and ASO size measured from left atrium [(26.07 +/- 5.32) cm vs. (25.91 +/- 5.32) cm] and right atrium [(22.13 +/- 5.31) cm vs. (22.08 +/- 5.26) cm, all P > 0.05]. The distances from simulation occluder to mitral valves [(5.76 +/- 2.39) cm] and to tricuspid valves [(8.25 +/- 2.40) cm] were similar as ASO to atria-ventricular valves [(5.61 +/- 2.26) cm and (8.02 +/- 2.48) cm, respectively, all P > 0.05].</p><p><b>CONCLUSIONS</b>The simulating percutaneous transcatheter closure of ASD by 3D visualization technique could be a helpful noninvasive approach for ASO selection before the procedure of transcatheter occlusion of ASD.</p>