ABSTRACT
Boron neutron capture therapy and Y-90 radioembolization are emerging therapeutic methods for uncontrolled brain cancers and hepatic cancers, respectively. These advanced radiation therapies are heavily relied on theranostic nuclear medicine imaging before the therapy for the eligibility of patients and the prescribed-dose simulation, as well as the post-therapy scanning for assessing the treatment efficacy. In Taiwan, the Taipei Veterans General Hospital is the only institute performing the BNCT and also the leading institute performing Y-90 radioembolization. In this article, we present our single institute experiences and associated theranostic nuclear medicine approaches for these therapies.
ABSTRACT
Boron neutron capture therapy and Y-90 radioembolization are emerging therapeutic methods for uncontrolled brain cancers and hepatic cancers, respectively. These advanced radiation therapies are heavily relied on theranostic nuclear medicine imaging before the therapy for the eligibility of patients and the prescribed-dose simulation, as well as the post-therapy scanning for assessing the treatment efficacy. In Taiwan, the Taipei Veterans General Hospital is the only institute performing the BNCT and also the leading institute performing Y-90 radioembolization. In this article, we present our single institute experiences and associated theranostic nuclear medicine approaches for these therapies.
Subject(s)
Humans , Boron Neutron Capture Therapy , Brain Neoplasms , Hospitals, General , Liver Neoplasms , Nuclear Medicine , Taiwan , Theranostic Nanomedicine , Treatment Outcome , VeteransABSTRACT
<p><b>OBJECTIVE</b>To observe the gene silencing and disruption of WNT pathway mediated by the specific shRNA targeted against beta-catenin and its effect on cell proliferation of the human colon cancer cell line Colo205.</p><p><b>METHODS</b>The shRNA plasmid vectors against beta-catenin were constructed and transfected into Colo205 cells with Lipofectamine 2000. The expression of beta-catenin was detected by RT-PCR and Western blot. Immunofluorescence staining was also performed to detect the beta-catenin protein expression in cells. The cell proliferation inhibition was determined by MTT assay and soft agar colony formation assay.</p><p><b>RESULTS</b>The shRNA vectors targeted against beta-catenin were successfully constructed and efficiently suppressed the expression of beta-catenin mRNA and protein(P<0.05). The expression inhibition rates were 47.89% and 45.26% at the mRNA and protein level respectively. Immunofluorescence microscopy also demonstrated the inhibition of beta-catenin protein induced by these specific shRNAs. The MTT assay indicated that the specific shRNA resulted in significant inhibition of cell growth on the culture plates in time-dependent manner. At 72 h post-transfection, the cell viability of CAT group was 48.5%, which was significantly different as compared with that of blank control group's 91.3%(P<0.05). In the soft agar, there were 9, 46, 43 cell colonies in the CAT, blank, and negative control groups respectively, which were significantly different(P<0.05).</p><p><b>CONCLUSIONS</b>The specific shRNAs targeted against beta-catenin has a gene silencing effect and blocks the WNT signaling pathway, which can inhibit the growth of Colo205 cells.</p>
Subject(s)
Humans , Cell Line, Tumor , Cell Proliferation , Colonic Neoplasms , Genetics , Metabolism , In Vitro Techniques , RNA Interference , Signal Transduction , Transfection , Wnt Proteins , Metabolism , beta Catenin , GeneticsABSTRACT
Effects of exercise on circulating thyroid hormone [TH] values remain controversial. We sought to observe the effect of treadmill exercise on serum TH values in highly selected subjects. Twenty-six healthy male military recruits aged 23-27 [mean, 25] years were studied. All had maintained identical diet and physical activity for a week before the test. Serum samples were drawn before [baseline] and immediately, 1, 4, 24, 24 and 48 h after maximal exercise [on a treadmill, Bruce protocol]. All subjects completed the protocol with normal ECG results. Specimens were analyzed to measure 3,3',5-triiodothyronine [T3], thyroxine [T4], free T4 [FT4], free T3 and thyroid-stimulating hormone [TSH] in the same assays. To determine the possible effect of hemodynamic changes, hematocrit [Hct]-adjusted data were also compared. Hemoconcentration, as reflected by increased Hct, was found immediately after exercise. No significant changes of serum mean TH values before and after exercise were found except for TSH, which increased significantly immediately after exercise [1.72 vs. baseline 1.42 IU/l, p < 0.01]. Values for T3, T4, and TSH increased significantly immediately after exercise, as compared to other postexercise values. However, the changes became insignificant after Hct adjustment. The FT4 values showed a reciprocal increase after exercise that became significant after Hct correction. Significantly negative correlation was found between FT4 and TSH values, but these values were still well within the normal range. Conclusions: Maximal treadmill exercise does not greatly affect the determination of concentrations of circulating THs