ABSTRACT
The fruits of Paulownia catalpifolia Gong Tong are used as a Chinese folk herbal medicine for the treatment of enteritis, tonsillitis, bronchitis, and dysentery, etc. Our previous study has identified new C-geranylated flavanones with obvious anti-proliferative effects in lung cancer A549 cells. In the present study, a new C-geranylated flavone, paucatalinone C (1) and five known C-geranylated flavanones (2-6) were isolated. In addition, a total of 34 C-geranylated flavonoids were detected by HPLC-DAD-ESI-MS/MS coupling techniques from the CHCl extract of P. catalpifolia. Futhermore, anti-aging effects of isolated compounds were evaluated in vitro with premature senescent 2BS cells induced by HO. Phytochemical results indicated that P. catalpifolia was a natural resource of abundant C-geranylated flavonoids. Diplacone (3) and paucatalinone A (5) were the potent anti-aging agents in the premature senescent 2BS cells induced by HO and the C-geranyl substituent may be an important factor because of its lipophilic character.
Subject(s)
Humans , Aging , Cell Line , Chromatography, High Pressure Liquid , Flavonoids , Chemistry , Pharmacology , Fruit , Chemistry , Hydrogen Peroxide , Toxicity , Magnoliopsida , Chemistry , Molecular Structure , Plant Extracts , Chemistry , Pharmacology , Tandem Mass SpectrometryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of the roots of Capparis tenera.</p><p><b>METHOD</b>The chemical constituents were isolated and repeatedly purified by kinds of column chromatography and the structures were elucidated by the NMR spectra and physicochemical properties.</p><p><b>RESULT</b>Eight compounds were isolated and identified as erigeside C (1), glucosuringic acid (2), vanillic acid 4-O-beta-D-glucoside (3-methoxy 4-glucosyl-benzoic acid) (3), 4-O-beta-D-glucopyranosylbenzoate (4), 3', 5'-dimethoxy- 4-O-beta-D-glucopyranosyl-cinnamic acid (5), tachioside (6), 2, 3-dihydroxy-1-(4-hydroxyl-3, 5-dimethoxyphenyl)-1-propanone (7) and acacetin 7-rutinoside (8).</p><p><b>CONCLUSION</b>Compounds 1-8 were all isolated from this plant for the first time and the compound 8 was isolated from this gene for the first time.</p>
Subject(s)
Capparis , Chemistry , Chromatography , Drugs, Chinese Herbal , Chemistry , Glycosides , Chemistry , Magnetic Resonance Spectroscopy , Plant Roots , Chemistry , Solubility , Water , ChemistryABSTRACT
<p><b>AIM</b>To study the bioactive constituents of the flower of Castanea mollissima Blume.</p><p><b>METHODS</b>Compounds were isolated and purified by column chromatography of silica gel and TLC. Structures were determined by various spectroscopic data, including IR, 1HNMR and 13CNMR, EIMS, FABMS and HMBC as well as comparison of the data with those reported in literatures.</p><p><b>RESULTS</b>Five compounds were isolated and elucidated as myricetin (I), quercetin (II), gallic acid (III), 4-quinolinone-2-caboxylic acid (IV), (+) -isolariciresinol-9'-O-alpha-L-rhamnoside (V).</p><p><b>CONCLUSION</b>These compounds were separated from the flower for the first time and compound V is a new compounds, named chestnutlignansoide.</p>
Subject(s)
Fagaceae , Chemistry , Flavonoids , Chemistry , Flowers , Chemistry , Molecular Structure , Naphthols , Chemistry , Plants, Medicinal , Chemistry , Quercetin , Chemistry , Rhamnose , ChemistryABSTRACT
<p><b>AIM</b>To study the bioactive constituents from the flower of Castanea mollissima Blume.</p><p><b>METHODS</b>Compounds were isolated and purified by column chromatography on silica gel and polyamide. Structures were determined by various spectroscopic data,including UV, IR, 1H and 13CNMR, EIMS and FABMS, 1H-13C-COSY and HMBC.</p><p><b>RESULTS</b>Two compounds were isolated from the ethyl acetate fraction of 95% ethanol extract and the structures were elucidated as kaempferol-3-O-[6"-(E)-p-coumaroyl]-alpha-D-mannopyranoside (1) and kaempferol-3-O-[6", 4"-di-(E)-p-coumaroyl]-alpha-D-mannopyranoside (2).</p><p><b>CONCLUSION</b>Compounds 1 and 2 are new compounds, named castanoside A and B respectirely.</p>