ABSTRACT
Objective:According to the GB/T 15000.3-2008， to develop a fucosterol certified reference material based on the project approved by Standardization Administration. Method:Fucosterol was isolated from <italic>Laminaria japonica</italic> dried thallus via 95% ethanol extraction， vacuum concentration， repeated column chromatography separation， recrystallization in petroleum ether-ethyl acetate， and residual solvent removal. Its chemical structure was identified by elemental analysis （EA）， infrared spectrum （IR）， mass spectrometry （MS）， nuclear magnetic resonance （NMR） and X-ray diffraction （XRD）. Its homogeneity， stability， and cooperative certification conducted by 8 laboratories were carried out by high performance liquid chromatography with evaporative light scattering detector. Result:For the fucosterol reference material， the certified value of purity was 99.54% with expanded uncertainty of 0.16% in confidence interval of 95%， the stability was good within 24 months storage period at 2-4 ℃， which met the technical requirements of reference material and passed the acceptance organized by Standardization Administration. Conclusion:The national standard materials of fucosterol has been successfully developed， which can be used for the determination of this component， the evaluation of detection methods， and the detection and quality control of related products.
ABSTRACT
Objective To explore the endoscopic Histoacryl injection in the treatment of gastric variceal hemorrhage based on the effect of psychological intervention. Methods 60 cases of patients with gastric variceal bleeding diagnosed in our hospital from January 2015 to March 2017 were randomly divided into control group and observation group according to the random number, 30 cases in each group.The control group was treated with endoscopic injection of tissue adhesive, the observation group were treated with endoscopic tissue adhesive and psychological care. The improvement of psychological status, treatment effect and prognosis were observed before and after treatment. Results The psychological state of patients in the observation group is better than control group, the treatment and the prognosis is better than the control group, there was statistical significant differences (P<0.05). Conclusion Endoscopic Histoacryl injection in the treatment of gastric variceal bleeding to the effect of psychological intervention can significantly. Fully improve the psychological state of patients, improve the clinical efficacy and prognosis, worthy of clinical application.
ABSTRACT
Objective To explore the endoscopic Histoacryl injection in the treatment of gastric variceal hemorrhage based on the effect of psychological intervention. Methods 60 cases of patients with gastric variceal bleeding diagnosed in our hospital from January 2015 to March 2017 were randomly divided into control group and observation group according to the random number, 30 cases in each group.The control group was treated with endoscopic injection of tissue adhesive, the observation group were treated with endoscopic tissue adhesive and psychological care. The improvement of psychological status, treatment effect and prognosis were observed before and after treatment. Results The psychological state of patients in the observation group is better than control group, the treatment and the prognosis is better than the control group, there was statistical significant differences (P<0.05). Conclusion Endoscopic Histoacryl injection in the treatment of gastric variceal bleeding to the effect of psychological intervention can significantly. Fully improve the psychological state of patients, improve the clinical efficacy and prognosis, worthy of clinical application.