ABSTRACT
Objective To establish a high performance liquid chromatography (HPLC) method for the determination of disodium edentate (EDTA-2Na) in nalmefene hydrochloride injection. Methods Content of EDTA-2Na in nalmefene hydrochloride injection was determined by HPLC. C18 Column was used. The mobile phase consisted of 0.3% tetrabutylammonium hydroxide solution-wateracetonitrile (20: 45: 35) with a flow rate of 1.0 ml/min, and the detection wavelength was 254 nm. The column temperature was 30°C and the injection volume was 20 µl. Results The quantification limit and detection limit were 0.199 and 0.060 µg/ml, respectively; the linear equation for the disodium edetate was Y=10.125X-0.216, and the calibration curves were linear within the range of 2.4593~ 7.3780 µg/ml (r=1.000). Each concentration of the average recovery was between 98~102% (n=9) (RSD%=0.58%). Conclusion The method can be used for the determination of EDTA-2Na in nalmefene hydrochloride injection, which is convenient, fast, sensitive and reproducible, with good precision, specificity and accuracy.
ABSTRACT
<p><b>OBJECTIVE</b>To compare the effect of different indications of blood transfusion on the treatment and complications in anemia of prematurity, and to investigate the suitable and reasonable indication of blood transfusion for our country.</p><p><b>METHODS</b>Ninety six hospitalized premature infants with birth weight less than 1500g and stay in hospital equal or more than 2 weeks were enrolled in the retrospective study and divided into two groups according to different indications of blood transfusion. There are 54 cases in observation group 1 (with strict indications for transfusion) and 42 cases in observation group 2 (with loose indications for transfusion).</p><p><b>RESULTS</b>In the observation group 1, the total amount of blood transfusion \[M(P25, P75):71.0(43.25, 107.25) ml\] and the times of blood transfusion \[3.00 (2.00, 4.00) times\] are both higher than those of the observation group 2 \[36.0 (29.50, 56.25) ml and 1.50 (1.00, 2.25) times, respectively, P = 0.00 1\]. The days of mechanical ventilation and the days of continuous positive airway pressure were more in the observation group 1, but oxygen supplement in the two groups had no significant difference. In observation group 1, the incidence of intracranial hemorrhage, fungal infection rate and mortality were higher than those of the observation group 2 (P < 0.05).</p><p><b>CONCLUSIONS</b>Anemia of prematurity easily led to the complications, and had obvious influence on the survival of premature infants. Clinician should consider concrete conditions of every patient before blood transfusion. It has positive significance on the survival of premature infants to loosen the indication properly and make the use of blood scientifically and rationally.</p>