ABSTRACT
<p><b>OBJECTIVE</b>To approach the treatment efficiency of replication defective adenovirus carrying HSV-tk gene under control of the human telomerase reverse transcriptase (hTERT) promoter in a mouse xenografts model of prostate cancer.</p><p><b>METHODS</b>It was erected that the model of human prostate cancer in BALB/C nude mouse followed by locally injecting Ad-hTERT-HSV-tk and peritoneal injection of GCV. The anti-tumor efficacy was evaluated by index of tumor volume, tumor weight, relative tumor curative, and tumor growth curve. Afterwards, the TUNEL and transmission electron microscope to overview apoptosis of tumor cells were used. Finally, immunohistochemistry for detecting PCNA of tumor cells were applied.</p><p><b>RESULTS</b>Compared with the control group, all viruses treatment groups demonstrated tumor growth inhibition. Among 3 treatment groups, antitumoral effect of Ad-hTERT-HSV-tk/GCV was much stronger than other two groups (P < 0.05). Obvious necrosis and apoptosis was observed by TUNEL, and PCNA was detected by immunohistochemistry in tumor tissues in all viruses treatment group.</p><p><b>CONCLUSIONS</b>Ad-hTERT-HSV-tk/GCV system is highly efficacious to inhabit the growth of human prostate cancer.</p>
Subject(s)
Animals , Female , Humans , Male , Mice , Adenoviridae , Genetics , Genetic Therapy , Methods , Genetic Vectors , Mice, Inbred BALB C , Mice, Nude , Promoter Regions, Genetic , Genetics , Prostatic Neoplasms , Pathology , Therapeutics , Recombination, Genetic , Telomerase , Genetics , Thymidine Kinase , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of epidermal growth factor (EGF) on estrogen receptor (ER) and androgen receptor (AR) in mouse prostate cells and explore the putative role of EGF in prostatic hyperplasia.</p><p><b>METHODS</b>Sixty male Kunming mice were randomly divided into two EGF groups and one control group (n=20) and subjected to subcutaneous injection of 1 and 2 microg/day EGF and distilled water, respectively, for 28 consecutive days. The cellular expression of ER and AR in the prostate of mice in different groups was evaluated by flow cytometry.</p><p><b>RESULTS</b>Compared with the control group, the positivity rate of ER and its expression level were significantly increased in the mouse prostate after EGF treatment (P<0.01), and the ER expression level was significantly higher in mouse with 2 microg/day EGF treatment than in those treated with 2 microg/day EGF (P<0.01). AR positivity rate and expression level also increased significantly in comparison with the control group (P<0.05), but no significant variation was found between 1 microg/day and 2 microg/day EGF groups.</p><p><b>CONCLUSION</b>EGF can increase the cellular expression of ER and AR in mice prostate and may play a role in the pathogenesis of prostatic hyperplasia.</p>
Subject(s)
Animals , Male , Mice , Epidermal Growth Factor , Pharmacology , Flow Cytometry , Injections, Subcutaneous , Prostate , Cell Biology , Metabolism , Prostatic Hyperplasia , Metabolism , Pathology , Random Allocation , Receptors, Androgen , Receptors, EstrogenABSTRACT
Objective To study the occurence, development and regulation of reactive gliosis with astrocyte (Ast) in vitro. Methods Ast was isolated and cultured in vitro and its model of reactive gliosis was established by scratching the cultured astrocytes. The reactivity and rules of Ast to injury was studied by morphological changes, RT-PCR, immunocytochemistry, in situ hybridization and imaging analysis. Results After scratching, the astrocytes showed typical features of reactive gliosis, with the hypertrophic cell body, thickened and lengtheded processes, and enhanced glial fibrillary acidic protein (GFAP) staining. In situ hybridization and RT-PCR analysis confirmed that the expression of GFAP mRNA was markedly increased. These changes occurred 1 d after scratching and reached the peak 5 to 7 d after injuring. Conclusion A model of reactive astrogliosis was successfully established in vitro which showed an active reaction to injury. The characteristics of reactive gliosis parallel that seen in vivo.
ABSTRACT
Objective To study the occurence, development and regulation of reactive gliosis with astrocyte (Ast) in vitro. Methods Ast was isolated and cultured in vitro and its model of reactive gliosis was established by scratching the cultured astrocytes. The reactivity and rules of Ast to injury was studied by morphological changes, RT-PCR, immunocytochemistry, in situ hybridization and imaging analysis. Results After scratching, the astrocytes showed typical features of reactive gliosis, with the hypertrophic cell body, thickened and lengtheded processes, and enhanced glial fibrillary acidic protein (GFAP) staining. In situ hybridization and RT-PCR analysis confirmed that the expression of GFAP mRNA was markedly increased. These changes occurred 1 d after scratching and reached the peak 5 to 7 d after injuring. Conclusion A model of reactive astrogliosis was successfully established in vitro which showed an active reaction to injury. The characteristics of reactive gliosis parallel that seen in vivo.