Preparation of mesoporous silica nanoparticles with different sizes and study on the correlation between size and toxicity / 药学学报

To investigate the crucial role of particle size in the biological effects of nanoparticles, a series of mesoporous silica nanoparticles (MSNs) were prepared with particle size gradients (50, 100, 150, 200 nm) with the traditional Stober method and adjusting the type and ratio of the silica source. The correlation between toxicity and size-caused biological effects were then further examined both in vitro and in vivo. The results indicated that the prepared MSNs had a uniform size, good dispersal, and ordered mesoporous structure. Hemolytic toxicity was found to be independent of particle size. At the cellular level, MSNs with smaller particle sizes were more readily internalized by cells, which initiated to more intense oxidative stress, therefor inducing higher cytotoxicity, and apoptosis rate. In vivo studies demonstrated that MSNs primarily accumulated in the liver and kidneys of mice. Pharmacokinetic analysis revealed that larger MSNs were eliminated more efficiently by the urinary system than smaller MSNs. The mice's body weight monitoring, blood tests, and pathological sections of major organs indicated good biocompatibility for MSNs of different sizes. Animal welfare and the animal experimental protocols were strictly consistent with related ethics regulations of Zhejiang Chinese Medical University. Overall, this study prepared MSNs with a particle size gradient to investigate the correlation between toxicity and particle size using macrophages and endothelial cells. The study also examined the biosafety of MSNs with different particle sizes in vivo and in vitro, which could help to improve the safety design strategy of MSNs for drug delivery systems.

Incidence of retinopathy of prematurity in Suzhou during 5a / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM:To analyze incidence and trends of retinopathy of prematurity(ROP)from 2011 to 2015 in Suzhou. <p>METHODS: A total of 2 527 infants were screened for ROP in Suzhou from January 2011 to December 2015 using RetCamII. First examination was performed from 4-6wk after birth. Of all the 2 527 infants, the birth weight was 640-4 200g with an average of 1694.99±370.875g. The gestational age were 24.5-41.1wk with an average of 32.397±2.2171wk. The ocular findings were recorded according to the International Classification of ROP and The Early Treatment for ROP. Only the more aggressive eye of bilateral asymmetrical cases was counted for statistical purpose. And the cases required surgeries(pre-threshold ROP type Ⅰ and threshold ROP)were defined as severe cases. <p>RESULTS: Of all the 2 527 infants, 408 infants were screened with ROP, in which 30 were severe ROP, the incidence of ROP and severe ROP was 16.15%and 1.19%. The incidence of ROP from 2011 to 2015 were 10.14%, 14.91%, 14.26%, 19.64%, 20.15% respectively, turned out a gradual upward trend(<i>χ²</i>=24.625, <i>P</i><0.01). The incidence of severe ROP were 1.18%, 0.94%, 0.63%, 0.90%, 2.22%, no significant differences between the groups(<i>P</i>>0.05). The incidence of ROP in the four seasons were 17.62%, 15.20%, 16.12%, 15.82% respectively and no significant differences between the groups(<i>χ²</i>=1.397, <i>P</i>=0.706). <p>CONCLUSION: The incidence of ROP in Suzhou increased from 2011 to 2015, which was related to the decline of the gestational age and birth weight. The distribution of ROP was not seasonal.

Prevalence of ROP in 3471 premature infants in Suzhou Municipal Hospital / 国际眼科杂志(Guoji Yanke Zazhi)

·AIM: To retrospectively analyze the prevalence of retinopathy of prematurity(ROP) in 3471 neonates in Suzhou Municipal Hospital. ·METHODS: A total of 3471 children (1947 males, 1524 females) were screened for ROP in Suzhou Municipal Hospital from January 2010 to September 2016 using binocular ophthalmoscope or ( and) RetCamⅡ. First examination was performed from 4-6wk after birth. The ocular findings were recorded according to the International Classification of ROP and The Early Treatment for ROP. Only the more aggressive eye of bilateral asymmetrical cases was counted for statistical purpose. Children with ROP in both binocular or single eye were counted in 1 case, and the cases required surgeries were defined as severe cases. The prevalence of ROP and severe ROP in recent 6a were analyzed retrospectively. ·RESULTS: The overall relevance ratio of ROP and severe ROP was 17.03% and 1.15%. The relevance ratio of ROP and severe ROP of the males were 16.38% and 1.08%,and of the females were 17.85% and 1.25%, the results were not statistically different (x2= 1. 296, P =0.255). The relevance ratio of ROP and severe ROP of the single birth infants were 17.61% and 1.13%, and of the multiple birth infants were 15.13% and 1.23%,the results were not statistically different (x2=2.706, P=0.100). The children were divided into 5 groups according to the birth weight. The relevance ratio of ROP with birth weight<1000g,1000-1499g,1500-1999g,2000-2499g and ≥2500g were 75. 00%, 36. 17%, 10. 75%, 6. 86% and 3. 77%respectively with significant differences (There were significant differences between the three groups which the birth weight <2000g, P<0.005). The relevance ratio of severe ROP were 36.54%, 1.68%, 0.31%, 0.19% and 0 respectively in these birth weight groups (There were significant differences between the three groups which the birth weight <2000g,P<0.005). The children were divided into 4 groups according to gestational weeks, the relevance ratio of severe ROP of gestational age<28wk,28-31wk, 32-36wk and ≥37wk were 69. 12%, 29. 91%, 8.28% and 3.33% respectively with significant differences (There were significant differences between the three groups which the gestational age <37wk, P<0.005). The relevance ratio of severe ROP were 25%, 1.52%, 0.24% and 0 in these gestational age groups respectively (There were significant differences between the three groups which the gestational age <37wk,P<0.005). · CONCLUSION: The detection rate of ROP in 3471 premature infants was 17. 03%, the severe ROP was 1.15%. There was no evidence that sex and birth were related to ROP, but lower birth weight and smaller gestational age increased the detection rate of ROP.

Relationship of intrauterine growth retardation and retinopathy of prematurity / 国际眼科杂志(Guoji Yanke Zazhi)

AIM:To analyze the association of intrauterine growth retardation (IUGR) and retinopathy of prematurity (ROP).METHODS:A retrospective analysis of a case series included in ROP screening from January 2011to December 2015 was performed in Suzhou Municipal Hospital.Totally 2527 children (5054 eyes) underwent screening.According to the gestational age,the data was divided into 4 groups (≤32wk,＞32 and ≤34wk,＞34 and ≤37wk,＞37wk).Every group was divided into two groups (IUGR group and no IUGR group) respectively.We compared the incidence of ROP in IUGR and non IUGR group.RESULTS:Of all the 2527 children,IUGR group were 702 including 78 ROP children,and non IUGR group were 1825 including 329 ROP children.There were 991 children were divided into ≤ 32wk group,including 63 IUGR in which 27 children were screened out ROP(42.9％) and 928 non IUGR in which 274 children were screened out ROP (29.5％),the difference on the incidence of ROP was statistically significant (X2 =4.958,P=0.026).There were 1025 children were divided into ＞ 32 and ≤ 34wk group,including 232 IUGR in which 33 children were screened out ROP(14.2％) and 793 non IUGR in which 51 children were screened out ROP (6.4％) and the difference was statistically significant (x2 =14.488,P＜0.001).There were 464 children were divided into ＞ 34 and ≤ 37wk group,including 374 IUGR in which 18 children were screened out ROP(4.8％) and 90 non IUGR in which 4 children were screened out ROP (4.4％) and the difference was not statistically significant (Fischer exact test,P=1).There were 47 children were divided into ＞37wk group,including 33 IUGR and 14 non IUGR,none were screened out in the two groups.CONCLUSION:Intrauterine growth retardation was closely related to the incidence of ROP.In the preterm infants with gestational age less than 34wk,the incidence of ROP in children with intrauterine growth retardation is significantly higher than that in children without intrauterine growth retardation.