ABSTRACT
As a new energy source for atrial fibrillation ablation, electric pulse ablation has higher tissue selectivity and biosafety, so it has a great application prospect. At present, there is very limited research on multi-electrode simulated ablation of histological electrical pulse. In this study, a circular multi-electrode ablation model of pulmonary vein will be built on COMSOL5.5 platform for simulation research. The results show that when the voltage amplitude reaches about 900 V, it can make some positions achieve transmural ablation, and the depth of continuous ablation area formed can reach 3 mm when the voltage amplitude reaches 1 200 V. When the distance between catheter electrode and myocardial tissue is increased to 2 mm, a voltage of at least 2 000 V is required to make the depth of continuous ablation area reach 3 mm. Through the simulation of electric pulse ablation with ring electrode, the research results of this project can provide reference for the voltage selection in the clinical application of electric pulse ablation.
Subject(s)
Humans , Heart Rate , Atrial Fibrillation/surgery , Electrodes , Catheter Ablation , ElectricityABSTRACT
Objective To develop a method of quantitative analysis of multi-components by single marker for nine effective constituents in Chenxiang-Huaqi tablets. Methods Using HPLC method, the mobile phase was acetonitrile -0.2% physcion acid in gradient elution program, the flow rate was 1.0 ml/min. The detection wavelength was set at 230 nm for liquiritin, ammonium glycyrrhizinate, costunolide and dehydrocostus lactone, and 283 nm for narirutin, naringin, hesperidin and neohesperidin, and 310 nm for pogostone. The column temperature was 30 and the injection volume was 10 μl.℃ Using Hesperidin as the internal standard, the relative correction factors among liquiritin, ammonium glycyrrhizinate, costunolide, dehydrocostus lactone, narirutin, naringin, neohesperidin, pogostone were detected by QAMS and their contents were calculated. The content difference of the above nine components with the external standard method were compared. Results The calculated values of hesperidin, liquiritin, ammonium glycyrrhizinate, costunolide, dehydrocostus lactone, narirutin, naringin, neohesperidin, pogostone were not statistical significance from those measured by external standard method (P>0.05). Conclusions The method offered reference for quality standard of Chenxiang-Huaqi tablets.
ABSTRACT
Objective@#To develop a method of quantitative analysis of multi-components by single marker for nine effective constituents in Chenxiang-Huaqi tablets.@*Methods@#Using HPLC method, the mobile phase was acetonitrile -0.2% physcion acid in gradient elution program, the flow rate was 1.0 ml/min. The detection wavelength was set at 230 nm for liquiritin, ammonium glycyrrhizinate, costunolide and dehydrocostus lactone, and 283 nm for narirutin, naringin, hesperidin and neohesperidin, and 310 nm for pogostone. The column temperature was 30 ℃ and the injection volume was 10 μl. Using Hesperidin as the internal standard, the relative correction factors among liquiritin, ammonium glycyrrhizinate, costunolide, dehydrocostus lactone, narirutin, naringin, neohesperidin, pogostone were detected by QAMS and their contents were calculated. The content difference of the above nine components with the external standard method were compared.@*Results@#The calculated values of hesperidin, liquiritin, ammonium glycyrrhizinate, costunolide, dehydrocostus lactone, narirutin, naringin, neohesperidin, pogostone were not statistical significance from those measured by external standard method (P>0.05).@*Conclusions@#The method offered reference for quality standard of Chenxiang-Huaqi tablets.