ABSTRACT
Objective To study the effect of interleukin (IL)-10 knockout (IL-10-/-) on renal repair after renal ischemia-reperfusion injury in mice.Methods Eighteen IL-10-/-mice (KO) aged 8-10 weeks and 18 C57BL/6 wild type mice (WT) aged 8-10 weeks were divided into control group (Sham) and renal ischemia-reperfusion injury (IRI) group.The renal tissue morphology change was observed by Hematoxylin and eosin (HE) staining and Masson staining.The expressions of IL-18, Ki67 and TGF-β1 were detected by immunohistochemistry.The expression of TGF-beta1 and IL-18 were detected by Western blotting.Results Compared with that in WT-IRI group, in KO-IRI group renal pathological damage was more severe, renal interstitial fibrosis was visible, Ki67 expression of renal tubular epithelial cells decreased distinctly (P＜0.01), the expression of TGF-betal increased significantly (P＜0.01).Conclusion Repair slows down significantly after kidney ischemia-reperfusion injury and fibrosis occurs gradually in IL-10-/-mice, eventually progressing to chronic kidney disease.
ABSTRACT
Objective: To study ginseng stem leaf saponin's effect on brain aging. Methods: The natural aging mice were adopted, divided randomly groups by weight and fed with ginseng stem leaf saponiu for three months, learning and memory ability of mice, the content of MDA and the action of MAO B of brain tissue were determined. The mice was fed with drugs for 40 days, the action of SOD and GSH Px of brain tissues were determined. Bissecting and fecthing the brain of the adult and aging rats and preparing brain chondriosomes and microsomes, and MDA content of brain chondriosomes and microsomes by the method of Fe 2+ cysteine inducing oxidation reduction reaction were determined. Results: Ginseng stem leaf saponin can increase learning and memory ability of aging mice, decrease the content of lipid peroxide and inhibit the action of MAO B of brain tissues; increase the action of SOD and GSH Px of brain tissues; and also inhibit the content of LPO induced oxidation reduction reaction by Fe 2+ cysteine in brain chondriosomes and microsomes of the adult and aging rats. Conclusion:Ginseng stem leaf saponin can protect brain function and postpone brain aging by decreasing free radical's damage and increasing action of GSH Px and SOD of brain tissue.