ABSTRACT
Aim To investigate the effect of Curcumin on the expression of HO-1 and Nrf-2,and explore the machanism of neuroprotection of Curcumin. Methods SH-SY5Y cells were treated with Curcumin at 0,1. 25, 5. 0,20. 0 ?mol?L -1,or with Curcumin at 5. 0?mol? L -1 for 12,24,48 h for the time course assay. RT-PCR and Western blot assays were carried out to detect mRNA and protein expression of Nrf-2 and HO-1. Nrf-2 siRNA was used to dectect the expression of HO-1 with the absence of Nrf-2. Results RT-PCR and Westernblot results indicated that the mRNA and protein expression of HO-1 and Nrf-2 all increased in a dose-and time-dependent manner after Curcumin treatment in the SH-SY5Y cells ( P
ABSTRACT
Aim To investigate the effects of Curcumin on Heme oxygenase isozymes in SH-SY5Y cells and explore a new mechanism of Curcumin in neuroprotection. Methods The human SH-SY5Y cells were cultured in vitro and treated with Curcumin at 0,1.25,5.0,20 ?mol?L-1 for 24 h,or with Curcumin at 5.0 ?mol?L-1 for 0,12,24,and 48 h. The active oxygen was detected by fluorescent probe DCFH-DA and fluorospectro-photometer. RT-PCR was used to detect the expression of HO-1 and HO-2 mRNA. Western blot was performed to detect the levels of HO-1 and HO-2 protein.Results The results showed that Curcumin could inhibit the levels of active oxygen(P