ABSTRACT
Objective@#To investigate the proper time of intratympanic steroid as combination therapy in treatment of patients with profound sudden sensorineural hearing loss(SSNHL) of total frequency type.@*Methods@#This prospective study included 50 patients with SSNHL in the First Affiliated Hospital of Chongqing Medical University from June 2017 to February 2018.All these patients had profound hearing loss averaged more than 81 dB at all frequencies (250-8 000 Hz). They were divided into two groups.The patients in group A were treated with simultaneous oral prednisone (1 mg/kg, qd) and intratympanic methylprednisolone (40 mg, qd) for 5 days.The patients in group B were treated with oral prednisone for 5 days as in group A, and then those who had no significant improvement were subsequently treated with intratympanic methylprednisolone (40 mg, qd)for further 5 days.All patients were treated with additional intravenous batroxobin and ginkgo biloba leaves extract.Following examination of pure tone audiogram, hearing gains and effective rates were statistically analyzed in both groups with SPSS software package(version 20.0).@*Results@#Hearings in both groups were improved significantly after treatment.In group A(20 cases), hearing gain was (29.2±22.7) dB and total effective rate was 65.0%, while in group B(22 cases), they were (27.3±22) dB and 68.2% respectively.There were no significant differences in hearing gain and recovery rate between two groups (hearing gain, P=0.793; total effective rate, P=0.827).@*Conclusions@#For patients with profound SSNHL of total frequency type, the efficacies of intratympanic steroid as simultaneous and subsequent therapy are equivalent. Therefore, subsequent intratympanic glucocorticoid is recommended for SSNHL patient of total frequency type to reduce complications and medical expenses.
ABSTRACT
Objective:We established nude mouse model of laryngocarcinoma and detect the disseminated tumor cell of peripheral blood and mutant p53 in tumour tissue,to research the correlation of DTC with variant p53 gene and their value of predicting cancer metastasis and prognosis in laryngocarcinoma.Methods:There are 40 nude mice in the experimental group which accepted hypodermic injection of laryngocarcinoma cell,10 in control.By the reverse transcription polymerase chain reaction and nested polymerase chain reaction(Nest-PCR),we tested CK19mRNA in nude mouse's peripheral blood,as a marker of disseminated cancer cell at the 10th weeks after tumor-planting.We also tested the expression of p53 gene in tumour tissue by RT-PCR and immunohistology.After the nude dead,we dissect lung,liver and lymph node and use continuous pathological dyeing confirmed whether the transfer.Results:The positive rate of DTC was 50.0%(20/40),but negative in the control group,the difference was significant(P