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OBJECTIVE@#The medicinal mushroom Sanghuangporus vaninii produces pharmaceutically valuable hispidin polyphenols in natural habitats. However, due to the slow growth in nature, S. vaninii grown in the field (sclerotia) is not reliable for pharmaceutical purposes. Although higher biomass of fungal mycelia can be obtained in submerged cultures, the accumulation of hispidin polyphenols is rare.@*METHODS@#In this study, the polyunsaturated fatty acids (PUFAs), linoleic acid (LA), linolenic acid (ALA), and methyl jasmonate (MeJa) were employed as the stimulant agents to coordinate the accumulation of biomass and hispidin polyphenols in its submerged cultures.@*RESULTS@#The addition of LA and ALA promoted the mycelial accumulation, while the addition of MeJa inhibited the growth of S. vaninii concomitant with reduced total polyphenols. UPLC-Triple-TOF-MS analysis revealed an increased production of hispidin, phellinstatin, pinnilidine, and its derivatives upon the addition of LA and ALA, and hypholomine B and its isomer, 3,14'-bihispidinyl, and phelligridin E upon the addition of MeJa on day 13. Intriguingly, total polyphenols from the MeJa-supplementing cultures harbored a high capacity in scavenging free radicals. Chemical structural analysis showed that hispidin polyphenols had higher antioxidant activity due to more hispidin moieties induced by MeJa.@*CONCLUSION@#The supplement of PUFAs affects the synthesis and composition of hispidin polyphenols in S. vaninii. Our results provide a possibility to coordinate the production of hispidin polyphenols via submerged cultures of S. vaninii.
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Objective To investigate the effects of using junior nurses in non-emergency department to take part in the training course of cardiopulmonary resuscitation (CPR) skills based on the defect situational simulation of crisis management. Methods A prospective randomly controlled test method was conducted, 66 junior nurses in non-emergency department newly-contracted in 2016 admitted to the Yuyao City People's Hospital from July 1, 2017 to Augest 30th, they were divided into a control group (n = 32) and an experimental group (n = 34) by random number table method. The nurses in control group received conventional CPR situational simulation teaching method, while the nurses in observation group received the training of using crisis management mode to direct realizing the defect situational simulation. The differences in the nurse team cooperation levels were compared between the two groups. Results After training, the team cooperation scores of the two groups of non-emergency department nurses were all higher than those before the training(control group: 99.78±12.33 vs. 92.34±12.35; experiment group:116.00±12.80 vs. 93.44±10.29, both P < 0.05), and the team cooperation score in observation group was significantly higher than that of control group (116.00±12.80 vs. 99.78±12.33, P < 0.01). Conclusion The defect situational simulation based on crisis management can effectively elevate the levels of CPR skills and team work cooperation in the non-emergency department junior nurses.
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Objective To investigate the association between genetic variants in microRNA biosynthesis genes and the risk of head and neck squamous cell carcinoma (HNSCC).Methods A case-control study was conducted with 576 HNSCC patients and 1 552 healthy controls matched by factors as age-(± 5 years) and sex.Eight potentially functional single nucleotide polymorphism loci in microRNA biosynthesis genes (DICER1,GEMIN3,and PIWIL1) were genotyped using the Illumina Infinium BeadChip platform.Univariate and multivariate logistic regression models were performed to assess the association between genotypes and HNSCC risk.Results The allele frequencies of rs1106042 (G＞A) in PIWIL1 were significantly different between the cases and controls (P=0.011).After controlling for factors as age,sex,smoking and alcohol intake,the A allele of rs 1106042 showed a decreased risk of HNSCC (additive model:adjusted OR=0.73,95％CI:0.57-0.93,P=0.011).Results from the stratification analysis by age,sex,smoking,alcohol intake and tumor sites showed that the effect of rs1106042 A allele on HNSCC risk was significant in older age groups (≥60),females,nonsmokers,non-alcohol drinkers,and subjects with oral cavity cancer (P＜0.05).Conclusion Potentially,functional single nucleotide polymorphism in PIWIL1 might modify the risk of HNSCC in China.
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Objective To investigate the association between genetic variants in microRNA biosynthesis genes and the risk of head and neck squamous cell carcinoma (HNSCC).Methods A case-control study was conducted with 576 HNSCC patients and 1 552 healthy controls matched by factors as age-(± 5 years) and sex.Eight potentially functional single nucleotide polymorphism loci in microRNA biosynthesis genes (DICER1,GEMIN3,and PIWIL1) were genotyped using the Illumina Infinium BeadChip platform.Univariate and multivariate logistic regression models were performed to assess the association between genotypes and HNSCC risk.Results The allele frequencies of rs1106042 (G＞A) in PIWIL1 were significantly different between the cases and controls (P=0.011).After controlling for factors as age,sex,smoking and alcohol intake,the A allele of rs 1106042 showed a decreased risk of HNSCC (additive model:adjusted OR=0.73,95％CI:0.57-0.93,P=0.011).Results from the stratification analysis by age,sex,smoking,alcohol intake and tumor sites showed that the effect of rs1106042 A allele on HNSCC risk was significant in older age groups (≥60),females,nonsmokers,non-alcohol drinkers,and subjects with oral cavity cancer (P＜0.05).Conclusion Potentially,functional single nucleotide polymorphism in PIWIL1 might modify the risk of HNSCC in China.
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<p><b>OBJECTIVE</b>We detected and analyzed the clinical values of total protein (TP), albumin (ALB), globulin (GLB), and ALB/GLB ratio (A/G) of whole unstimulated saliva of healthy people to determine the time of day when saliva composition is relatively stable. We compared the protein concentration and A/G of whole unstimulated saliva of patients with chronic periodontitis with those of healthy volunteers to provide references for diagnostic methods and clinical applications of saliva.</p><p><b>METHODS</b>The whole saliva of 37 healthy subjects were collected at 8:00, 9:30, 11:30, 13:00, 16:30, and 21:00. Meanwhile, the whole saliva of 24 patients with periodontitis was collected in the morning. Bicinchoninic acid method was used to detect the TP content. Saliva ALB was detected by GF-D800 semi-automatic biochemical analyzer, and the GLB and A/G were calculated. Finally, the results were statistically analyzed using SPSS 19.0.</p><p><b>RESULTS</b>Salivary protein compositions were stable in the morning on an empty stomach. Healthy people: TP, (1 354.35±389.52) µg.mL-1; ALB, (139.55±27.19) µg.mL-1; GLB, (1 211.80±360.73) µg.mL-1; A/G, 0.126 3±0.041 7. Subjects with chronic periodontitis: TP, (2 611.56±231.62) µg.mL-1; ALB, (296.27±17.34) µg.mL-1; GLB, (2 315.69±221.67) µg.mL-1; A/G, 0.156 2±0.017 3. The contents of TP, ALB, and GLB in healthy individuals at different periods within a day showed significant differences (P<0.05), which were mainly reflected in the levels before and after meals. No significant difference was detected in A/G. The concentrations of TP, ALB, and GLB were significantly increased in patients with chronic periodontitis compared with those in healthy volunteers. However, no significant difference existed in A/G.</p><p><b>CONCLUSION</b>Salivary protein compositions are more stable in the morning than in other periods. Thus, mornings can be set as the time of specimen collection in future research. The concentrations of TP, ALB, and GLB in patients with chronic periodontitis are higher than those in healthy people.</p>
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Humans , Albumins , Chronic Periodontitis , Globulins , SalivaABSTRACT
<p><b>OBJECTIVE</b>To investigate whether programmed death ligand 1 (PD-L1) expressed in the periodontal tissue of chronic periodontitis and the correlativity of PD-L1 and different degrees of chronic periodontitis, provide experience for immunoregulation mechanism, clinical treatment and prognosis of chronic periodontitis.</p><p><b>METHODS</b>Gingiva and periodontal tissue of healthy people and chronic periodontitis patients were collected. Based on clinical probing, periodontal tissue were classified into three groups: periodontal tissues of healthy people, periodontal tissue of mild chronic periodontitis, periodontal tissue of severe chronic periodontitis. Fluorescent quantitation polymerase chain reaction was applied to explore the expression of PD-L1 mRNA in the periodontal tissue of the different groups. Western blot and immunohistochemistry method were utilized to test the expression of PD-L1 protein in the periodontal tissue of the different groups. Combining with clinical image data, the relationship between differentially expressions of PD-L1 and different degrees of chronic periodontitis was analyzed.</p><p><b>RESULTS</b>The relative expression quantity of PD-L1 in the periodontal tissue of the mild chronic periodontitis was significantly higher that of the severe chronic periodontitis (P<0.01). The relative expression quantity of PD-L1 in the periodontal tissue of healthy subjects and severe chronic periodontitis had no statistical significance (P>0.05).</p><p><b>CONCLUSION</b>The expression of PD-L1 in the periodontal tissue negativelv regulates inflammatory periodontal tissue damage.</p>