Effects of Flow Components C6 and C7 in N-butyl Alcohol Extract from the Leaves of Cestrum nocturnum on the Proliferation and Apoptosis of Human Gastric Cancer Cell SGC7901 / 中国药房

OBJECTIVE:To study the effects of flow components C6 and C7 in n-butyl alcohol extract from the leaves of Ces-trum Nocturnum(CN)on the proliferation and apoptosis of human gastric cancer cell SGC7901. METHODS:C6 and C7 were ob-tained by using different ratio of chloroform and methanol(1:9,1:7)to the gradient elution of CN leaves. After cultured with 0 (blank control),5,10,20,40,80 μg/ml C6 and C7 for 72 h,inhibitory effect of C6 and C7 on the proliferation of SGC7901 was determined by MTT assay. Inhibitory rate and IC50 were calculated. After SGC7901 were cultured with 10 μg/ml C6 and C7 for 72 h,colony formation assay was utilized to detect the effects of C6 and C7 on the cell colony formation,and the rate of colony for-mation was calculated. In addition,Wright/Giemsa and Hoechst33258/PI staining assay were used to observe the change of cytomor-phology. RESULTS:MTT showed that C6 and C7 had inhibitory effect on the proliferation of SGC7901 to different extent;inhibi-tory rates were 22.1%-80.0% and 19.6%-79.7%,and IC50 were 16.4,18.05 μg/ml,respectively. Compared with blank control group,colony formation rate of C6 and C7 group decreased,with statistical significance(P<0.05). The number of apoptotic cells was more in treatment group than in other groups. CONCLUSIONS:Flow components C6 and C7 in n-butyl alcohol extract from the leaves of CN can inhibit the proliferation of SGC7901 cells and induce the apoptosis of them.

Study on pharmacokinetics and relative bioavailability of rapid oral disintegrating tablet of dimenhydrinate / 第二军医大学学报

Objective:To compare the pharmacokinetics and relative bioavailability of rapid oral disintegrating tablet of dimenhydrinate(RODTD) and those of market available tablet of dimenhydrinate(DMH).Methods: Eight healthy volunteers were evenly randomized into 2 groups,one group received RODTD(25 mg) and the other received available market tablet of dimenhydrinate(25 mg).The blood levels of DMH were determined by high performance liquid chromatography(HPLC) before and after drug administration in 2 groups.Chromatography conditions were: Nova-Pak C_(18)as chromatographic column, methanol triethylamine buffer((11),)flow rate: 1.0 ml/min,detection wavelength: 225 nm,and room temperature.The pharmacokinetics and relative bioavailability of RODTD and market available tablets were investigated.Results: The standard curve of DMH in the blank plasma was linear within the range of 5-500 ng/ml,with the regression equation being C=0.004 4 A+4.745 and R~(2)=(0.996.)The limit of detection was 2 ng/ml;the average recovery rate was(90.55?4.69)% and the RSD was 0.041%.The intra-day derivations of 3 different concentrations(low,middle,and high) of plasma were 9.27%,4.93%,and 2.95%,respectively((n=5),) and the inter-day derivations were 9.97%,3.81%,and 3.06%,respectively(n=5).Blood samples(3 ml) were subjected to HPLC assay and significant difference was found between the 2 forms of DMH. The pharmacokinetic parameters of RODTD were: AUC=(602.04?113.82) ng?h?ml~(-1),C_(max)=(95.86?21.28) ng?ml~(-1),and T_(Peak)=(1.8?0.32) h;the pharmacokinetic parameters of market available tablets were:AUC=(342.73?84.96) ng?h?ml~(-1),C_(max)=((46.34?)(10.32)) ng?ml~(-1),and T_(Peak)=(2.65?0.24) h.Statistical analysis showed there was significant difference in the relative bioavailability of 2 forms of DMH(P