ABSTRACT
This work was to isolate and identify the bioactive secondary metabolite which was produced by myxobacteriumStigmatella eracta WXNXJ-B, and to evaluate its antitumor and apoptosis-inducing effects. The results showed that one novel compound [molecular formula C29H25NO3] was isolated, purified by Sephadex LH-20 column chromatography and preparative RP-HPLC, and identified as 5-[6-benzyl-quinolin-3-ylmethyl]-6- phenyl-3,7-dioxa- bicycle [4.1.0] heptan-3-one [named as quinoxalone] according to its UV, IR, HRMS and NMR spectra. The compound showed strong antitumor activity on B16, HepG2, MCF-7, SGC-7901, MDA-MB231 and CT-26 six tumor cell lines in vitro. Nevertheless, it showed less cytotoxic to the mouse normal spleen cells [IC50 was 836.27 +/- 13.02 mg mL-1]. The cytotoxic study on HepG2 cellsin-vitro showed that quinoxalone could induce the change of cell nuclear and arrested the cell division in the S and G2/M phase. Our results suggest that quinoxalone could be a potential anti-cancer agent
ABSTRACT
Objective: To detect the contents of the active components in five kinds of Chinese crude herbs and Samples Avoidable for Boiling(SAB). Methods: With modern technology, astraglin in Radix Astragali, atractylons and butenolide in Rhizoma Atractylodis Macrocephalae, paeonol in Cortex Moutan , sterols in Radix Morindae Officinalis and flavonoids in Radix Puerariae were detected and analyzed. Results: In SAB, the contents of astraglin, atractylone, butenolide, paeonol and sterols were low, especially the high molecular polysaccharides and volatile active components of atractylone, butenolide and paeonol. Meanwhile, more impurities were found in SAB. Conclusion: There are some defects in the production techniques and quality control of SAB.
ABSTRACT
Objective: To study the antitumor effect of the protein from Tricholoma matsutake against HeLa cells in vitro. Methods: Active protein was separated from the mycelium after sub-merged fermentation, and the antitumor effect against HeLa cells was studied in vitro by cell growth inhibition, scanning electron microscope, flow cytometry and DNA electrophoresis. Results: The mycelium protein-TMP-B had strong antitumor effect and induced apoptosis in vitro. TMP-B inhibited cell proliferation and induced cell apoptosis by arresting cell conversion from S to G 2/M state. HeLa cells exposed to TMP-B showed apoptotic peaks. Agarose gel electrophoresis of DNA from cells treated with TMP-B revealed "DNA ladder". Conclusion: The mycelium protein from Tricholoma matsutake can inhibit cervix cancer cells--HeLa cell proliferation and induce apoptosis.