A STUDY OF BONE AND VASCULAR CHANGE IN FABRICATING THE NEO-OSSEOUS FLAP USING ILIAC BONE AND INFERIOR EPIGASTRIC VESSEL

Generally fabrication of neovascularized bone flap are produced by snadwiching vessels between bone segment with the optimal size and shape for the recipient sites. When these flaps are transferred to the recipient site, they are again isolated from the surrounding soft tissues, resulting not only decrease in the blood flow to bone segments by sacrifices of peripheral blood circulation due to elevation of flap, but also possible changes of shape of osseous flap by bone resorption. To overcome these disadvantage, it may be considerable to block bone segment off from the surrounding soft tissue. Purpose of this study are observed time-dependent changes of blood flow, vascularity and histologic finding of bone flaps using the autogenous bone and local vessel implantation. The results are compared with bone flap which had not been blocked off from surrounding soft tissues. Neovascularized bone flap were fabricated in 90 Sprague-Dawley rats using autogenous iliac bone and superficial inferior epigastric vessels. The experimental flaps were wrapped with silastic sheets to block neovascularization from surrounding soft tissues, and the control flaps were left unwrapped. The degree of bone formation, vascularity and blood flow were then assessed at 1, 2, 4, 6 and 8 weeks after flap fabrication, using a histological examination, microangiogram, corrosion cast study and radioactive microspheres. When fabricating a neovascularized bone flap using the autogenous iliac bone and the superficial inferior epigastric vessels, the physico-histological organization, vascularity and blood flow of neovascularized flaps were same or superior for wrapped than for unwrapped bone segments until the 4th week after grafting. This means that the period of neovascularization can be lengthened by the 4th weeks in the fabrication of bone segments, which can be blocked off from surrounding soft tissues. For these reasons, blocked bone flap fabrication can be considered to be superior to the conventional method.

A case report of pneumocephalus after total maxillectomy

No abstract available.

Prevalence of intestinal helminthic infections and skin tests for Paragonimus and Clonorchis in tuberculosis patients

This study was conducted to evaluate prevalence of intestinal helminthic infections and skin tests and Paragonimus and Clonorchis in tuberculosis(TB) patients in Korea. Stool examinations by formalin-ether technique and intradermal tests were carried out in 2 areas; Masan TB hospital and Kong-Ju TB hospital. The positive rate of intestinal helminths of any kind was 51.7 percent among 329 patients in Masan hospital, but 18.1 percent among 215 patients in Kong-Ju hospital. The infection rates of Trichocephalus trichiurus and Clonorchis sinensis were 20.7 percent and 17.6 percent respectively in Masan hospital patients, but the infection rates of T. trichiurus and C. sinensis were 6.5 percent and 6.0 percent respectively in Kong-Ju hospital patients. In skin tests, positive reactions to Paragonimus and Clonorchis antigens were 22.0 percent and 37.6 percent respectively in Masan hospital patients. On the contrary, Kong-Ju hospital patients showed the positive rates of 15.2 percent for Paragonimus and 27.2 percent for Clonorchis respectively.

Toxoplasma antibodies by indirect latex agglutination tests in St. Mary's Hospital patients

A total of 421 patients hospitalized in St. Mary's Hospital were examined by indirect latex agglutination test in order to evaluate the Toxoplasma antibody in Korean from June to August 1981. The test sera of the patients were obtained from each age group by random sampling. The 421 samples of test sera showed negative in 153, 1:2 in 157, 1:4 in 59, 1:8 in 27, 1:16 in 7, 1:32 in 9, 1:64 in 2, 1:128 in 4 and 1:256 in 3 cases, respectively. The positive rate of Toxoplasma antibody was 4.3 percent in this sample when indirect latex antibodies of 1:32 or higher were regarded as positive. The titers of positive Toxoplasma antibodies were increased by age.

A comparative analysis of various parts of Ascaris suum with respect to their protein composition

For the purpose of making a comparative study of protein compositions in Ascaris suum by sexes and body parts, extracts were prepared from whole bodies, body walls, genital organs, digestive organs and body fluid, of both sexes. And electrophoretic analysis was conducted using polyacrylamide slab gel in the presence of sodium dodecyl sulfate. The results were as follows: In this study, protein bands of each part were separated in the largest number and most clearly under 8 -12 percent (10 percent) gradient gel condition. The number of bands by body parts was 43 in body walls, 51 in genital organs, 47 in digestive organs, and 34 in body fluid. When examined in terms of sex, the number of bands of whole body was 38 in females and 35 in males. The electrophoretic patterns of body wall protein were in most cases similar with those seen in digestive organs. And the band with a molecular weight of 72,900 was unique to the body wall, and the 122,000 MW band was unique to the female body wall. In genital organ extracts, large molecular weight proteins (more than 80,000) were more frequently met. The molecular weights showed some differences between the two sexes. Of the bands, those having molecular weights of 119,700, 100,500, 88,500 and 86,100 were unique to the female genital organs. On the other hand, the male genital organs showed unique bands having molecular weights of 87,100, 82,800, and 15,500. An unique band common to the genital organs of both sexes was one having 49,300 MW. In the digestive organs evenly distributed protein bands of molecular weights of l0,000-120,000 were observed. The band having 59,800 MW was unique to the digestive organs. The number of bands obtained from body fluid was comparatively small, and the number of bands having less than 30,000 MW was 7, accounting for 55 percent of the total protein amounts. The bands having 47,600 MW and 31,400 MW were unique to body fluid.

Analysis of protein components at varioue stages of Clonorchis sinensis

In this study the authors examined the protein components at various stages of Clonorchis sinensis, and those of tegument and metabolite of adult Clonorchis by using SDS-polyacrylamide gel electrophoresis and immunodiffusion. The following results were obtained: The protein components of C. sinensis were gradually changed during its development. A considerable change occurred during the initial 7 days after the metacercarial infection. Two bands of protein of about 97,000 molecular weight (MW) and 178,000 MW were unique to excysted metacercaria of C. sinensis. Other 2 bands of protein of 23,000 and 25,000 MW which were absent in metacercariae, might be associated with the development of sex organs in adult. In the metabolite, some components of tegumental proteins were detected. And this tegumental protein components in metabolite seems to be the major antigenic components reacting with infected rabbit antiserum by immunodiffusion. Twenty bands of protein were detected in the isolated adult tegument. Among them 6 bands were in 97,000-65,000 MW, 3 bands in 56,000-53,000 MW and 5 bands in 37,000-30,000 MW. On the other hand, in metabolic products of adult C. sinensis, 17 bands were detected.