ABSTRACT
Objective: To investigate the anti-gout effect of hirudin and its mechanism. Methods: Hypoxanthine was used to replicate mouse model of hyperuricemia. Sodium uric induced acute gouty inflammation in rats to observe the effect of hirudin on the level of uric acid and inflammation induced by acute hyperuricemia. The serum uric acid, serum urea nitrogen, serum xanthine oxidase activity, and liver xanthine oxidase activity were observed in chronic hyperuricemia mouse model induced by potassium oxonate. The changes of renal pathology, the level of C-reactive protein (CRP) and the expression of glucose transporter 9 (GLUT9) were also be tested. Results: Hirudin could significantly reduce the serum uric acid level in hyperuricemia mice induced by hypoxanthine and significantly inhibit acute toe swelling induced by sodium uric acid in rats. It could significantly reduce the level of serum hyperuricemia, decrease the level of blood urea nitrogen (BUN), significantly inhibit the expression of GLUT9 and alleviate the pathological changes of kidney in chronic hyperuricemia mice induced by potassium oxazinate. Conclusion: Hirudin has significant anti-hyperuricemia and anti-gout effects, and its mechanism may be related to the protection of kidney and the regulation of GLUT9 expression.
ABSTRACT
Objective: To observe the therapeutic effects on gout and the acute toxicity of Poecilobdella manillensis. Methods: The anti-gout effects of P. manillensis were studied by observing its effects on the hyperuricacidemia model and the level of blood uric acid. And we observed the effect of P. manillensis on gouty inflammation via injection of MSU to the foot of rats. The analgesic effect and toxicity of P. manillensis will be reported in this work too. Results: Comparing with the model group, P. manillensis could significantly reduce the level of the blood uric acid in the hyperuricemic and normal mice at 3.00 and 1.50 g/kg doses (P < 0.01), and acute gouty arthritis at 0.75 g/kg dose (P < 0.05, 0.01). It reduced the number of writhing in mice at 3.00 and 0.75 g/kg doses (P < 0.05, 0.01). Obvious toxic reaction was not observed and the maximum tolerant dose was 23.09 g/kg for mice in acute toxicity experiment. Conclusion: P. manillensis is proved to have the therapeutic effect on gout. Further research and development will be continued.