A study of global ecological adaptability and field selection practices of Panax ginseng / 中国中药杂志

Through the development of ecological suitability analysis of producing area and the selection criteria of farmland cultivation in the global range of ginseng, we aim to provide scientific basis for rational planning, production layout and standardized planting of farmland. We analyze the data based on the ecological factors from 271 sample plots of Panax ginseng, including both the traditional producing regions recorded in past dynasties medicinal works and the popular production regions in the world, using global geographic information system for medicinal plant(GMPGIS) developed by ICMM (Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences). We concluded that the suitable producing areas in global for P. ginseng mainly included America, Canada, China, Russia, Japan, North Korea, France, Italy, Ukraine, and South Korea. In addition, the suitable producing areas in China mainly included Heilongjiang, Jilin, Liaoning, Shanxi, Gansu, Hubei, Sichuan, Inner Mongolia, Shandong, and Shanxi. Besides, based on the references and the experience of ginseng-producing and our many years' work on the 1,000-hectare plantation of P. ginseng, we established a standard land selection protocol for cultivation of P. ginseng. The use of GMPGIS to select the most optimum ginseng production regions provides a new scientific basis for introduction, cultivation, tending, protection, cultivation normalization for P. ginseng and the standard land selection protocol would lay a solid foundation for the high quality P. ginseng production.

Anti-proliferation and radiosensitization effects of chitooligosaccharides on human lung cancer line HepG2

Objective: To observe the anti-proliferation and radiosensitization effect of chitooligosaccharides (COS) on human lung cancer cell line HepG2. Methods: CCK-8 assay was employed to obtain the inhibition ratio of COS on HepG2 cells at 24 h after treatment. The clonogenic assay was used to analyze the cell viability of RAY group and RAY + COS group with X-ray of 0, 1, 2, 4, 6 and 8 Gy, and the cell survival curve was used to analyze the sensitization ratio of COS. Flow cytometry was employed to detect cell cycle and apoptosis rate in control group, RAY group and RAY + COS group after 24 h treatment. Results: COS inhibited the proliferation of HepG2 cells, and the inhibition rate positively correlated with the concentration of COS. The cell viability decreased with increasing exposure dose in RAY group and RAY + COS group. The cell viabilities of RAY + COS group were lower than those of RAY group at the dose of 4, 6 and 8 Gy (P < 0.05), and the sensitization ratio of COS was 1.19. There were higher percentage at G

Anti-proliferation and radiosensitization effects of chitooligosaccharides on human lung cancer line HepG2

OBJECTIVE@#To observe the anti-proliferation and radiosensitization effect of chitooligosaccharides (COS) on human lung cancer cell line HepG2.@*METHODS@#CCK-8 assay was employed to obtain the inhibition ratio of COS on HepG2 cells at 24 h after treatment. The clonogenic assay was used to analyze the cell viability of RAY group and RAY + COS group with X-ray of 0, 1, 2, 4, 6 and 8 Gy, and the cell survival curve was used to analyze the sensitization ratio of COS. Flow cytometry was employed to detect cell cycle and apoptosis rate in control group, RAY group and RAY + COS group after 24 h treatment.@*RESULTS@#COS inhibited the proliferation of HepG2 cells, and the inhibition rate positively correlated with the concentration of COS. The cell viability decreased with increasing exposure dose in RAY group and RAY + COS group. The cell viabilities of RAY + COS group were lower than those of RAY group at the dose of 4, 6 and 8 Gy (P < 0.05), and the sensitization ratio of COS was 1.19. There were higher percentage at G2/M phase and apoptosis rate, and lower percentage at S phase in RAY + COS group versus the other two groups (P < 0.01).@*CONCLUSIONS@#COS can inhibit the proliferation of HepG2 cells, and enhance the radiosensitization of HepG2 cells, induce apoptosis and G2/M phase arrest.

The interaction between microsomal epoxide hydrolase polymorphisms and indoor pollution in non small cell lung cancer / 中华预防医学杂志

<p><b>OBJECTIVE</b>To determine the interaction between indoor air pollution and mEH gene polymorphisms.</p><p><b>METHODS</b>Blood samples from 222 non small cell lung cancer patients and 222 healthy people were characterized by PCR and PCR-RFLP methods. The interaction coefficients were determined through unconditional logistic regression model.</p><p><b>RESULTS</b>Significant differences in the positive rate of mEH-exon3 mutant and the heterozygote were found between case and control groups (chi(2) = 7.046, P = 0.030). But no significant difference was found in mEH-exon4 non-wild-type between groups (chi(2) = 2.674, P = 0.263). mEH-exon3 mutant (OR = 1.99; 95% CI = 1.21, 3.25) could significantly increase the risk of lung cancer. After adjusted by confounding variables, significant interactions were found between the use of coal-wall stove and the non-wild type mEH gene. The interaction coefficients were increased with the duration of exposure and quantity of coal consumed. The super multiplication models were established between non-wild type mEH gene and the exposure to soot or oil fume during cooking. The interaction coefficients were 2.75 and 7.34 respectively for exon3 and exon4. No interaction was found between non-wild type mEH gene and irritation of eye or throat during cooking.</p><p><b>CONCLUSION</b>Through the molecular epidemiological techniques, we confirmed indoor air pollution that caused by coal burning was a noticeable lung cancer risk factor. The interaction between the polymorphisms of mEH gene and the indoor air pollution plays an important role in the carcinogenesis of lung.</p>

Analysis of CT features of pulmonary sarcoidosis / 中华放射学杂志

Objective To investigate the CT features of pulmonary sarcoidosis.Methods Ninety patients with histologically proved pulmonary sarcoidosis were retrospectively studied by using CT scans and clinical recording.Results The main CT findings of pulmonary sarcoidosis were nodules which were seen in 69 cases(76.7%),and the nodules mostly distributed around the bronchovascular bundle(n=37, 41.1%).Other abnormalities included consolidation(n=31,34.4%),ground-grass(n=39,43.3 %), thickening of bronchovascular bundle(n=30,33.3%),interlobular septal lines(n=58,64.4%), fibrosis(n=17,18.9%)including bronchial distortion(n=8,8.9%),linear shadow(n=5,5.6%), and honeycombing shadow(n=4,4.4%),air-trapping(n=3,5.3%),bronchial straitness(n=8, 8.9%),pleural thickening(n=42,46.7%),and hilar and mediastinal adenopathy(n=76,84.4%). Two or more abnormal findings co-existed in 83 cases.The pulmonary lesions co-existed with hilar and mediastinal adenopathy in 76 cases.The nodules(n=25),consolidation(n=9),ground-grass(n=11), thickening of bronehovascular bundle(n=10)were improved after therapy.Ten cases of the interlobular septal(10/22),0 of bronchial distortion(0/4),1 case of diffuse linear(1/3),and 0 case of honeycombing(0/2)were improved.Conclusion CT manifestations of pulmonary sarcoidosis are varied, but has some specific radiographic features.A correct diagnosis can be made.combined with hilar and mediastinal adenopathy.