ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of decoction for invigorating the kidney and improving blood circulation to thrombosis and pathology on rabbit blood stasis model.</p><p><b>METHOD</b>Thirty rabbits were ramdomly divided into normal group, model group, high dose group, low dose group and Xue Shuan Ning group. Tissue-type plasminogen activator (t-PA), plasminogen activator inhibitor (PAI), fibrinogen (Fbg) and D-dimer (DD) were investigated after those rabbits had been treated. One rot was solected randomly from each group to observe pathological changes.</p><p><b>RESULT</b>There were significant differences in t-PA, PAI, Fbg and DD between normal group and other groups is very obvious (P < 0.01) . Between groups of high dose low dose Xue Shuan Ning and model, the statistical differeces were significant, as well as between groups of high dose, low dose and Xue Shuan Ning groups (P < 0.05). However, there was no statistical difference between high dose group and high dose group (P > 0.05). The pathological changes in model group were most serious, those in Xue Shuan Ning were less serious. There were slight pathological changes in high dose group and low dose group.</p><p><b>CONCLUSION</b>Models ware made successfully. High dose group and low dose group have stronger effect on thrombosis than Xue Shuan Ning group.</p>
Subject(s)
Animals , Female , Male , Rabbits , Blood Viscosity , Dose-Response Relationship, Drug , Drug Combinations , Drugs, Chinese Herbal , Pharmacology , Fibrin Fibrinogen Degradation Products , Metabolism , Fibrinogen , Metabolism , Hematocrit , Plants, Medicinal , Chemistry , Plasminogen Inactivators , Blood , Random Allocation , Thrombosis , Blood , Pathology , Tissue Plasminogen Activator , BloodABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of decoction for invigorating the kidney and improving blood circulation to thrombosis on rabbits blood stasis model.</p><p><b>METHOD</b>Thirty rabbits were randomly divided into normal group, model group, heavy dose group, slight dose group and xue shuan ning group. Tissue-type plasminogen activator (t-PA), plasminogen activator inhibitor (PAI), fibrinogen (Fbg) and D-dimer (DD) were investigated after those rabbits had been treated. One was selected randomly from each group to observe pathological changes.</p><p><b>RESULT</b>There was significant difference in t-PA, PAI, Fbg and DD between normal group and other groups (P < 0.01). Among groups of heavy dose, slight dose, xue shuan ning and model, the statistical differences were significant, as well as among groups of heavy dose, slight dose and xue shuan ning (P < 0.05). However, there was no statistical difference between heavy dose group and slight dose group (P > 0.05). The pathological changes in model group were most serious, and those in xue shuan ning were less serious. There were slight pathological change in heavy dose group and light dose group.</p><p><b>CONCLUSION</b>Models were made successfully. Heavy dose group and slight dose group have stronger effect on thrombosis than xue shuan ning group.</p>
Subject(s)
Animals , Female , Male , Rabbits , Dose-Response Relationship, Drug , Drug Combinations , Drugs, Chinese Herbal , Pharmacology , Fibrin Fibrinogen Degradation Products , Metabolism , Fibrinogen , Metabolism , Kidney , Pathology , Liver , Pathology , Lung , Pathology , Medicine, Chinese Traditional , Plants, Medicinal , Chemistry , Plasminogen Inactivators , Blood , Random Allocation , Thrombosis , Blood , Pathology , Tissue Plasminogen Activator , BloodABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effects of Yi-Shen-Huo-Xue Fang on expression of GMP-140 and cleaning out the oxygenic free radicle on rabbits blood stasis model.</p><p><b>METHOD</b>Thirty rabbits were divided randomly into five groups as the normal group, model group, large dose of "Yi-Shen-Huo-Xue Fang" group, small dose of "Yi-Shen-Huo-Xue Fang" group and "Xue-Shuan-Xin-Mai-Ning" group. After being treated respectively, granule membrane protein 140(GMP-140), erythrocyte sueroxide dismutase (E-SOD), erythrocyte lipid peroxide(E-LPO), plasma lipid peroxide(P-LPO) were checked up.</p><p><b>RESULT</b>The GMP-140, E-SOD, E-LPO, P-LPO in normal control were compared with those in model groups, With the difference(P < 0.01), model control group was compared with large dose group and small dose group (P < 0.01), with "Xue-Shuan-Xin-Mai-Ning" group(P < 0.05), large dose group was compared with "Xue-Shuan-Xin-Mai-Ning" group(P < 0.05), and large dose group were compared with small dose group (P > 0.05).</p><p><b>CONCLUSION</b>The model was made successfully. Large dose group, small dose group and "xue-shuan-xin-mai-ning" group can inhibit expression of GMP-140, enhence SOD activity and decrease LPO content on blood stasis rabbit model. Large dose group and small dose group have stronger effect than "xue-shuan-xin-mai-ning" group.</p>