Icariin alleviates lipid peroxidation after spinal cord injury in rats / 南方医科大学学报

<p><b>OBJECTIVE</b>To assess the effects of intragastric administration of icariin on lipid peroxidation after spinal cord injury in rats.</p><p><b>METHODS</b>Seventy-two healthy adult male SD rats were randomized equally into icariin group, control group and sham-operated group. In the control and icariin groups, spinal cord injury was induced using modified Allen's method, and the rats in the sham-operated group underwent laminotomy without damaging the spinal cord. Immediately after the surgery, the rats in icariin group were subjected to intragastric administration of icariin (100 mg/kg), and those in the control and sham-operated groups received an equal volume of saline in the same manner once a day. At 24 h after the operation, malondialdehyde (MDA) content was detected using thiobarbituric acid method, superoxide dismutase (SOD) activity was measured with xanthine oxidase method, and the water content in the spinal cord was measured using dry-wet weight method. At 48 h after the operation, the ultrastructure of the spinal cord was observed with transmission electron microscopy and scored using Kaptanoglu scoring method. The motor function of the rats was assessed using BBB scoring at 7, 14, 21 and 28 days after the operation.</p><p><b>RESULTS</b>At 24 h after the operation, MDA content was significantly higher in the control group and icariin group than in the sham-operated group, and was significantly lower in icariin group than in the control group (P<0.05); SOD activity was significantly higher in icariin group than in the control group, and was both significantly lower than that in the sham-operated group (P<0.05). At 48 h after operation, the water content and ultrastructure score of the spinal cord were the highest in sham-operated group (P<0.05), and were significantly lower in icariin group than in the control group (P<0.05). At all the time points of measurement, the BBB scores were significantly lower in the control and icariin groups than in the sham-operated group (P<0.05), and were significantly higher in icariin group than in the control group (P<0.05).</p><p><b>CONCLUSION</b>Icariin can significantly reduce MDA content, increase SOD activity, and ameliorate lipid peroxidation, spinal cord edema, and histopathological damage of the spinal cord to improve motor function of rats with spinal cord injury.</p>

Neuroprotective effect of icariin on spinal cord injury in rats / 中国骨伤

OBJECTIVE@#To study the neuroprotective effect of icariin on spinal cord injury in rats.@*METHODS@#A total 108 SPF male 3-month-old SD rats were divided into experimental group, control group and sham operation group according to the random number table. There were 36 rats in each group. In the control group and the experimental group, the modified Allen's method was used to make the spinal cord injury model. In the sham operation group, only the lamina was cut without damaging the spinal cord. Immediately after operation, the experimental group was given intragastric administration of icariin(100 mg/kg), the control group and sham operation group were given an equal amount of normal saline by gavage, twice a day. BBB score was used to assess the motor function of rats on 1, 2, 3 days after operation. At 72 h after operation, the activity of myeloperoxidase (MPO) was measured by spectrophotometry. Tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) levels was detected by enzyme-linked immunosorbent assay(ELISA). MPO, TNF-α, IL-1β expression were detected by immunohistochemical staining. Malondialdehyde (MDA) content was detected by thiobarbituric acid method. Superoxide dismutase (SOD) activity was measured by xanthine oxidase method. TUNEL staining was used to detect the apoptosis and apoptosis index(AI) was calculated. The histopathological changes of the spinal cord were observed under a light microscope and the histopathological score was performed using Sirin score method.@*RESULTS@#BBB score in the control group and the experimental group was significantly lower than that in the sham operation group at each postoperative time point(<0.05). BBB score in the experimental group was significantly higher than that in the control group at 2 and 3 days after operation (<0.05). At 72 h after operation, the MPO activity and the levels of TNF-α, IL-1β in the control group and experimental group were significantly higher than in the sham operation group (<0.05), and the experimental group was obviously higher than control group(<0.05). The expressions of MPO, TNF-α, IL-1β in the control group and experimental group were significantly higher than in the sham operation group (<0.05), and the experimental group was significantly lower than of the control group (<0.05). MDA content in the control group and the experimental group was significantly higher than that in the sham operation group, and the experimental group was significantly lower than that in the control group (<0.05). SOD activity in the control group and the experimental group was significantly lower than that in the sham operation group, and the experimental group was significantly higher than that in the control group (<0.05). The AI in the control group and the experimental group was significantly higher than that in the sham operation group, and the experimental group was significantly lower than that in the control group (<0.05). The histopathological score in the control group and the experimental group was significantly higher than that in the sham operation group, and the experimental group was significantly lower than that in the control group (<0.05).@*CONCLUSIONS@#Icariin can inhibit inflammation, lipid peroxidation and apoptosis after spinal cord injury, reduce histopathological damage of spinal cord, improve the motor function, effectively protect spinal cord tissue, and has an obvious neuroprotective effect.

Percutaneous laser disc decompression for cervical vertigo / 中华外科杂志

<p><b>OBJECTIVE</b>To investigate the curative effect and mechanism of transcutaneous laser disc decompression for the treatment of cervical vertigo.</p><p><b>METHODS</b>From October 2000 to October 2004, 42 patients with cervical vertigo were treated with percutaneous laser disc decompression by applying a Nd: YAG laser (wavelength is 1064 nm). The postoperative follow-up period was more than 2 months, the change of patients' vertigo were observed.</p><p><b>RESULTS</b>All the patients were followed up. The mean follow-up period was 7.5 months (from 2 to 36 months). After 2 months of postoperative, 28 patients' vertigo disappeared (67%), 6 patients' vertigo improved (14%), 8 patients' vertigo did not improve. The effective rate was 81%, there was no complication (infection and nerve injury).</p><p><b>CONCLUSIONS</b>Cervical intervertebral disc protrusion and cervical spine instability irrigate the neck sympathetic nerve, result in the spasm of vertebral artery, which is the main cause of cervical vertigo. Percutaneous laser disc decompression can decrease intradiscal pressure, increase local temperature, remove the spasm of the vertebral artery. The therapeutic effect for the treatment of cervical vertigo was remarkable.</p>

Reform Clinical Education and Train Creative Students with High Quality / 中华医学教育探索杂志

Traditional clinical students training model is teacher-centered this education model has some defects.Whether an education model is advanced or not is relative to whether we can cultivate creative students with high quality.We use“one center, two auxiliarys,three stages”in our clinical education and fully utilize advanced education methods,combine theory with practice and cultivate creative students wiht high quality.